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Volume 49, Issue 3

The Photoassimilation of Organic Compounds by Autotrophic Blue-green Algae Free

Abstract

SUMMARY

Four obligately photoautotrophic blue-green algae were shown to assimilate acetate. This reaction was light dependent and was greatly decreased in the absence of carbon dioxide. Acetate was incorporated mainly into the ethanol extractable (lipid) fraction of the organisms and into the protein fraction. Only four amino acids (glutamate, proline, arginine, leucine) were significantly radioactive as a result of the incorporation of (1-) or (2-)C-acetate. Partial degradations of amino acids derived from specifically labelled acetate and enzyme assays on crude cell-free extracts of support the operation of established pathways for the biosynthesis of these amino acids. Growth of blue-green algae was not significantly affected by the presence of moderate concentrations of the sodium salts of the lower fatty acids, with the exception of propionate, low concentrations of which inhibited growth.

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1967-12-01
2024-04-19
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References

  1. Albrecht A. M., Vogel H. J. 1964; Acetyl ornithine δ-transaminase. Partial purification and repression behaviour. J. biol. Chem 239:1872
     [Google Scholar]
  2. Allison F. E., Hoover S. R., Morris J. H. 1937; Physiological studies with the nitrogen fixing alga Nostoc muscorum . Botan. Gaz 98:433
     [Google Scholar]
  3. Allison R. K., Skipper H. E., Reid M. R., Short W. A., Hogan G. L. 1953; The assimilation of acetate by Nostoc muscorum . J. biol. Chem 204:197
     [Google Scholar]
  4. Archibald R. M. 1944; Determination of citrulline and allantoin and demonstration of citrulline in blood plasma. J. biol. Chem 156:121
     [Google Scholar]
  5. van Baalen C. 1965a; Quantitative surface plating of coccoid blue-green algae. J. Phycol 1:19
     [Google Scholar]
  6. van Baalen C. 1965b; Aldolase in blue-green algae. Nature, Lond 206:193
     [Google Scholar]
  7. Baich A., Pierson D. J. 1965; Control of proline biosynthesis in Escherichia coli. Biochim . Biophys. Acta 104:397
     [Google Scholar]
  8. Benson A. A., Bassham J. A., Calvin M., Good ale T. C, Haas W., Stepka W. 1950; Path of carbon in photosynthesis : V. Paper chromatography and radioautography of the products. J. Am. chem. Soc 72:1710
     [Google Scholar]
  9. Bray G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analyt. Biochem 1:279
     [Google Scholar]
  10. Buchanan B. B., Evans M. C. W. 1965; The synthesis of α-ketoglutarate from succinate and carbon dioxide by a sub-cellular preparation of a photosynthetic bacterium. Proc. natn. Acad. SciU.S.A 541212
     [Google Scholar]
  11. Buchanan B. B., Evans M. C. W. 1966; The synthesis of phosphoenol pyruvate and ATP by extracts of photosynthetic bacteria. Biochem. biophys. Res. Comm 22:484
     [Google Scholar]
  12. Butler R. G., Umbreit W. W. 1966; Absorption and utilization of organic matter by the strict autotroph Thiobacillus thiooxidans, with special reference to aspartic acid. J. Bact 91:661
     [Google Scholar]
  13. Carr N. G., Pearce J. 1966; Photoheterotrophism in blue-green algae. Biochem. J 99:28
     [Google Scholar]
  14. Cheung W. Y., Gibbs M. 1966; Dark and photometabolism of sugars by a blue-green alga: Tolypothrix tenuis . Plant Physiol 41:731
     [Google Scholar]
  15. Chinard F. P. 1952; Photometric estimation of proline and ornithine. J. biol. Chem 199:91
     [Google Scholar]
  16. Cutinelli C, Ehrensvard G., Reio L., Saluste E., Stjernholm R. 1951; Acetic acid metabolism in Escherichia coli. I. General features, and the metabolic connection between acetate and glutamic acid, aspartic acid, glycine, alanine, valine, serine and threonine. Acta chem. scand 5:353
     [Google Scholar]
  17. Delwiche C. C., Finstein M. S. 1965; Carbon and enzyme sources for the nitrifying autotroph Nitrobacter. J. Bact 90:102
     [Google Scholar]
  18. Dixon G. H., Kauffman D. L., Neurath H. 1958; Amino acid sequence in the region of di- isopropylphosphoryl binding in diisopropylphosphoryl trypsin. J. biol. Chem 233:1373
     [Google Scholar]
  19. Evans M. C. W., Buchanan B. B. 1965; Photoreduction of ferredoxin and its use in carbon dioxide fixation by a subcellular system from a photosynthetic bacterium. Proc. natn. Acad. SciU.S.A 531420
     [Google Scholar]
  20. Evans M. C. W., Buchanan B. B., Arnon D. I. 1966; A new ferredoxin-dependent carbon dioxide reduction cycle in a photosynthetic bacterium. Proc. natn. Acad. SciU.S.A 55928
     [Google Scholar]
  21. Fay P. 1965; Heterotrophy and nitrogen fixation in Chlorogloea fritschii . J. gen. Microbiol 39:11
     [Google Scholar]
  22. Fewson C. A., Al-Hafidh M., Gibbs M. 1962; Role of aldolase in photosynthesis. I. Enzyme studies with photosynthetic organisms with special reference to blue-green algae. Plant Physiol 37:402
     [Google Scholar]
  23. Friedmann T. E. 1957; Determination of α-keto acids. Meth. Enzymol 3:414
     [Google Scholar]
  24. Harder R. 1917; Ernährungsphysiologische Untersuchungen an Cyanophyceen, hauptsachlich dem endophytischen Nostoc. punctiforme . Z. Bo tan 9:145
     [Google Scholar]
  25. Hill C. H. 1952; Studies on the inhibition of growth of Streptococcus faecalis by sodium propionate. J. biol. Chem 199:329
     [Google Scholar]
  26. Hoare D. S. 1963; The breakdown and biosynthesis of glutamic acid. J. gen. Microbiol 32:157
     [Google Scholar]
  27. Hoare D. S., Gibson J. 1964; Photoassimilation of acetate and the biosynthesis of amino acids by Chlorobium thiosulphatophilum . Biochem. J 91:546
     [Google Scholar]
  28. Hoare D. S., Moore R. B. 1965; Photoassimilation of organic compounds by autotrophic blue-green algae. Biochem. biophys. Acta 109:622
     [Google Scholar]
  29. Hoare D. S., Hoare S. L., Moore R. B. 1966a; Photosynthesis of amino acids from acetate in an autotrophic blue-green alga. Proc. Am. Soc. Microbiol73
     [Google Scholar]
  30. Hoare D. S., Hoare S. L., Moore R. B. 1966b; Photosynthesis of amino acids from acetate by blue-green algae. Abstr. int. Congr. Microbiol 9:157
     [Google Scholar]
  31. Hoare D. S., Hoare S. L. 1966; Feedback regulation of arginine biosynthesis in blue-green algae and photosynthetic bacteria. J. Bact 92:375
     [Google Scholar]
  32. Holm-Hansen O., Brown G. W. 1963; Ornithine cycle enzymes in the blue-green alga Nostoc muscorum . Plant and Cell Physiol 4:299
     [Google Scholar]
  33. Hudock G. A. 1962; Formation of N-acetyl glutamate in Chlamydomonas reinhardii. Biochem . biophys. Res. Comm 9:551
     [Google Scholar]
  34. Ida S., Alexander M. 1965; Permeability of Nitrobacter agilis to organic compounds. J. Bact 90:151
     [Google Scholar]
  35. Jones M. E., Lipmann F. 1955; Aceto-Co-A-kinase. Meth. Enzymol 1:585
     [Google Scholar]
  36. Kelly D. P. 1965a; Assimilation of organic compounds by a strictly chemoautotrophic Thiobacillus. J. gen. Microbiol 41:v
     [Google Scholar]
  37. Kelly D. P. 1965 b; Influence of organic compounds on Thiobacillus. Biochem. J 100:9
     [Google Scholar]
  38. Kemble A. R., McPherson H. T. 1954; Determination of monoamino monocarboxylic acids by quantitative paper chromatography. Biochem. J 56:548
     [Google Scholar]
  39. Kiyohara T., Fujita Y., Hattori A., Watanabe A. 1960; Heterotrophic culture of a blue-green alga, Tolypothrix tenuis . J. gen. appl. Microbiol 6:176
     [Google Scholar]
  40. Kratz W. A., Myers J. 1955a; Nutrition and growth of several blue-green algae. Am. J. Bo tan 42:282
     [Google Scholar]
  41. Kratz W. A., Myers J. 1955b; Photosynthesis and respiration of three blue-green algae. Plant Physiol 30:275
     [Google Scholar]
  42. Lipmann F., Tuttle L. C. 1945; A specific micromethod for the determination of acyl phosphates. J. biol. Chem 159:21
     [Google Scholar]
  43. Maas W. K., Novelli G. D., Lipmann F. 1953; Acetylation of glutamic acid by extracts of Escherichia coli . Proc. natn. Acad. SciU.S.A 391004
     [Google Scholar]
  44. Moore B. G., Tischer R. G. 1965; Biosynthesis of extracellular polysaccharides by the blue-green alga Anabaena flos-aquae . Can. J. Microbiol II:877
     [Google Scholar]
  45. Myers J., Clark L. B. 1944; Culture conditions and the development of the photosynthetic mechanism. II. An apparatus for the continuous culture of Chlorella. J. gen. Physiol 28:103
     [Google Scholar]
  46. Nichols B. W., Harris R. V., James A. T. 1965; The lipid metabolism of blue-green algae. Biochem. biophys. Res. Comm 20:256
     [Google Scholar]
  47. Oginsky E. L. 1957; Isolation and determination of arginine and citrulline. Meth. Enzymol 3:639
     [Google Scholar]
  48. Pearce J., Carr N. G. 1966; Enzymes of acetate metabolism in blue-green algae. J. gen. Microbiol 45:i
     [Google Scholar]
  49. Ravel J. M., Humphreys J. S., Shive W. 1965; Control of glutamine synthesis in Lactobacillus arabinosus . Arch. Biochem. Biophys 111:720
     [Google Scholar]
  50. Richter G. 1961; The lack of diphosphofructose aldolase in two photosynthetic organisms: Anacystis nidulans and Rhodopseudomonas spheroides . Biochim. biophys. Acta 48:606
     [Google Scholar]
  51. Roberts R. B., Cowie D. B., Abelson P. H., Bolton E. T., Britten R. J. 1955; Studies of biosynthesis in Escherichia coli . Publ. Carneg. Instn. No. 607
     [Google Scholar]
  52. Sadler W. R., Stanier R. Y. 1960; The function of acetate in photosynthesis by green bacteria. Proc. natn. Acad. SciU.S.A 461328
     [Google Scholar]
  53. Spector L., Lipmann F. 1955; Carbamyl phosphate, the carbamyl donor in enzymatic citrulline synthesis. J. Am. chem. Soc 77:819
     [Google Scholar]
  54. Stadtman E. R. 1957; Preparation and assay of acyl coenzyme A and other thiolesters; use of hydraxylamine. Meth. Enzymol 3:931
     [Google Scholar]
  55. Still G. C., Wang C. H. 1965; The role of the Krebs cycle reactions in the biosynthetic functions of Thiobacillus thioparus . Fedn Proc, Fedn Am. Socs exp. Biol 24657
     [Google Scholar]
  56. Stross R. G. 1960; Growth response of Chlamydomonas and Haematococcus to the volatile fatty acids. Can. J. Microbiol 6:611
     [Google Scholar]
  57. Taylor T. G. 1953; A modified procedure for the microestimation of citrate. Biochem. J 54:48
     [Google Scholar]
  58. Warburg O., Christian W. 1942; Isolierung und Kristallization des Garungsferments Enolase. Biochem. Z 310:384
     [Google Scholar]
  59. Webster G. C., Frenkel A. W. 1953; Some respiratory characteristics of the blue-green alga, Anabaena. Plant Physiol 28:63
     [Google Scholar]
  60. Wiessner W. 1964; Direct and indirect photometabolism of acetate in green algae. Plant Physiol 39:XIV
     [Google Scholar]
  61. Willard J. M, Schulman M., Gibbs M. 1965; Aldolase in Anacystis nidulans and Rhodo- pseudomonas spheroides . Nature, Lond 206:195
     [Google Scholar]
  62. Yemm E. W., Cocking E. C. 1954; The determination of amino acids with ninhydrin. Analyst 80:209
     [Google Scholar]
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The Photoassimilation of Organic Compounds by Autotrophic Blue-green Algae
Microbiology 49, 351 (1967); https://doi.org/10.1099/00221287-49-3-351
/content/journal/micro/10.1099/00221287-49-3-351
/content/journal/micro/10.1099/00221287-49-3-351
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