%0 Journal Article %A Robinson, G. L. %T Laboratory Cultivation of Some Human Parasitic Amoebae %D 1968 %J Microbiology, %V 53 %N 1 %P 69-79 %@ 1465-2080 %R https://doi.org/10.1099/00221287-53-1-69 %I Microbiology Society, %X SUMMARY: Cultural conditions and supplementary substances necessary for the laboratory cultivation of some human parasitic amoebae were investigated by using a basal solution containing inorganic salts, citrate and lactate. Three supplementary components were found necessary: starch grains, animal protein (soluble or insoluble) and living bacteria. Restraint of bacterial growth by antibiotics, and of the development of an alkaline reaction (due to pellicle-forming aerobes) by carbon dioxide, improved the amoebic growth. Under these conditions, all the common parasitic amoebae examined, except Ioda-moeba butschlii, were grown and maintained for long periods in laboratory culture. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-53-1-69