Laboratory Cultivation of Some Human Parasitic Amoebae Robinson, G. L.,, 53, 69-79 (1968), doi = https://doi.org/10.1099/00221287-53-1-69, publicationName = Microbiology Society, issn = 1350-0872, abstract= SUMMARY: Cultural conditions and supplementary substances necessary for the laboratory cultivation of some human parasitic amoebae were investigated by using a basal solution containing inorganic salts, citrate and lactate. Three supplementary components were found necessary: starch grains, animal protein (soluble or insoluble) and living bacteria. Restraint of bacterial growth by antibiotics, and of the development of an alkaline reaction (due to pellicle-forming aerobes) by carbon dioxide, improved the amoebic growth. Under these conditions, all the common parasitic amoebae examined, except Ioda-moeba butschlii, were grown and maintained for long periods in laboratory culture., language=, type=