RT Journal Article SR Electronic(1) A1 Robinson, G. L.YR 1968 T1 Laboratory Cultivation of Some Human Parasitic Amoebae JF Microbiology, VO 53 IS 1 SP 69 OP 79 DO https://doi.org/10.1099/00221287-53-1-69 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Cultural conditions and supplementary substances necessary for the laboratory cultivation of some human parasitic amoebae were investigated by using a basal solution containing inorganic salts, citrate and lactate. Three supplementary components were found necessary: starch grains, animal protein (soluble or insoluble) and living bacteria. Restraint of bacterial growth by antibiotics, and of the development of an alkaline reaction (due to pellicle-forming aerobes) by carbon dioxide, improved the amoebic growth. Under these conditions, all the common parasitic amoebae examined, except Ioda-moeba butschlii, were grown and maintained for long periods in laboratory culture., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-53-1-69