@article{mbs:/content/journal/micro/10.1099/00221287-57-2-273, author = "BROWN, JANE E. and BROWN, P. R. and CLARKE, PATRICIA H.", title = "Butyramide-utilizing Mutants of Pseudomonas aeruginosa 8602 which Produce an Amidase with Altered Substrate Specificity", journal= "Microbiology", year = "1969", volume = "57", number = "2", pages = "273-285", doi = "https://doi.org/10.1099/00221287-57-2-273", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-57-2-273", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY Mutants of Pseudomonas aeruginosa 8602 were isolated which, unlike the wild type, were able to grow with butyramide as a carbon source. Six mutants derived from the constitutive strain c 11 were shown to produce an enzyme (B amidase) with altered electrophoretic mobility and altered substrate specificity. The apparent K m for butyramide of the B amidase was about a tenth of that of the A amidase and the V max was about ten-fold greater. A further mutation produced mutants able to grow on valeramide.", }