f A Simple Chemically Defined Medium for the Production of Phase I Bordetella pertussis
- Authors: D. W. Stainer, M. J. Scholte
- Microbiology, October 1970 63: 211-220, doi: 10.1099/00221287-63-2-211
- Subject: Physiology And Growth
- Published Online:
SUMMARY: A simple, chemically defined medium is described consisting of sodium glutamate, proline, cystine, salts, and growth factors, which is suitable for the large-scale production of phase I Bordetella pertussis. More than 30 x 109 organisms/ml. were produced in 48 to 72 h. growth in shake flasks and fermentors. The cultures were detoxified by the addition of 0.14% formalin to yield vaccines which were non-toxic to mice and guinea pigs and had good mouse-protective antigen levels. The antigenic stability was satisfactory after storage of the final vaccines at elevated temperatures.
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/content/journal/micro/10.1099/00221287-63-2-211dcterms_title,dcterms_subject-pub_serialIdent:journal/micro AND -contentType:BlogPost64
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