A Simple Chemically Defined Medium for the Production of Phase I Bordetella pertussis
Stainer, D. W. and Scholte, M. J.,, 63, 211-220 (1970),
doi = https://doi.org/10.1099/00221287-63-2-211,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= SUMMARY: A simple, chemically defined medium is described consisting of sodium glutamate, proline, cystine, salts, and growth factors, which is suitable for the large-scale production of phase I Bordetella pertussis. More than 30 × 109 organisms/ml. were produced in 48 to 72 h. growth in shake flasks and fer- mentors. The cultures were detoxified by the addition of 0·14 % formalin to yield vaccines which were non-toxic to mice and guinea pigs and had good mouse-protective antigen levels. The antigenic stability was satisfactory after storage of the final vaccines at elevated temperatures.,
language=,
type=