RT Journal Article SR Electronic(1) A1 Stainer, D. W. A1 Scholte, M. J.YR 1970 T1 A Simple Chemically Defined Medium for the Production of Phase I Bordetella pertussis JF Microbiology, VO 63 IS 2 SP 211 OP 220 DO https://doi.org/10.1099/00221287-63-2-211 PB Microbiology Society, SN 1465-2080, AB SUMMARY: A simple, chemically defined medium is described consisting of sodium glutamate, proline, cystine, salts, and growth factors, which is suitable for the large-scale production of phase I Bordetella pertussis. More than 30 × 109 organisms/ml. were produced in 48 to 72 h. growth in shake flasks and fer- mentors. The cultures were detoxified by the addition of 0·14 % formalin to yield vaccines which were non-toxic to mice and guinea pigs and had good mouse-protective antigen levels. The antigenic stability was satisfactory after storage of the final vaccines at elevated temperatures., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-63-2-211