1887

Abstract

SUMMARY: A method for the selection of auxotrophic mutants in using -glycosyl-polifungin has been developed. The method results in about 9000-fold enrichment. All types of auxotrophs were obtained. Under certain conditions preferential selection of nucleic acid hydrolysate-requiring auxotrophs was observed. The frequency of occurrence of these mutants was about 50% of all colonies tested. Cysteine auxotrophs of were obtained for the first time by means of this method.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-84-1-111
1974-09-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/84/1/mic-84-1-111.html?itemId=/content/journal/micro/10.1099/00221287-84-1-111&mimeType=html&fmt=ahah

References

  1. Chuen-Chin H., Feingold D. F. 1973; Polyene antibiotic action on leuthin liposomes: effect of cholesterol and fatty acyl chains. Biochemical and Biophysical Research Communications 51:972–978
    [Google Scholar]
  2. Davis B. D. 1948; Isolation of biochemically deficient mutants of bacteria by penicillin. Journal of the Amercan Chemical Society 70:4267
    [Google Scholar]
  3. Ditchburn P., MacDonald K. D. 1971; The differential effects of nystatin on growth of auxotrophic and prototrophic strains of Aspergillus nidulans . Journal of General Microbiology 67:299–306
    [Google Scholar]
  4. Kinsky S. C., Luse S. A., van Deenen L. L. M. 1966; Interaction of polyene antibiotics with natural and artificial membrane systems. Federation Proceedings 25:1503–1510
    [Google Scholar]
  5. Kotiuszko D., Wituch K., Morawska H., Siejko D. 1971; Polifungin, a new antifungal antibiotic. I. Morfological, cultural and physiological properties of Streptomyces sp. strain producing a new complex of tetraene antibiotics. Acta microbiologica polonica 3–4135–143
    [Google Scholar]
  6. Lampen J. O., Arnow P. M., Borowska Z., Laskin L. 1962; Location and role of sterol at nystatin binding sites. Journal of Bacteriology 84:1152–1160
    [Google Scholar]
  7. Lederberg J., Zinder N. 1948; Concentration of biochemical mutants of bacteria with penicillin. Journal of the American Chemical Society 70:4267
    [Google Scholar]
  8. Moat A. G., Peters N. Jun., Srb A. M. 1959; Selection and isolation of auxotrophic yeast mutants with the aid of antibiotics. Journal of Bacteriology 7:673–677
    [Google Scholar]
  9. Norman A. W., Demel R. A., de Kruyff B., van Kessel G. W. S. M., van Deenen L. L. M. 1972; Studies on the biological properties of polyene antibiotics: comparison of the other polyenes with filipin in their ability to interact specifically with sterol. Biochimica et biophysica acta 290:1–14
    [Google Scholar]
  10. Pieniążek N. J., Bal J., Balbin E., Stepień P. P. 1974; Two pathways for cysteine biosynthesis in Aspergillus nidulans . Molecular and General and Genetics (in the press)
    [Google Scholar]
  11. Pieniazek N. J., Stępień P. P., Paszewski A. 1973; An Aspergillus nidulans mutant lacking cystathionine β-synthase: identity of l-serine sulfhydrylase with cystathionine β-synthase and its distinctness from O-acetyl-l-serine sulfhydrylase. Biochimica et biophysica acta 297:37–47
    [Google Scholar]
  12. Pontecorvo G., Roper J. A., Hemmons L. M., MacDonald K. D., Bufton A. W. J. 1953; The genetics of Aspergillus nidulans . Advances in Genetics 5:141–238
    [Google Scholar]
  13. Snow R. 1966; Enrichment method for auxotrophic mutants using the antibiotic nystatin. Nature, London 211:206–207
    [Google Scholar]
  14. Stachiewicz E., Quostel J. H. 1963; Amino acid transport in yeast and effect of nystatin. Canadian Journal of Biochemistry and Physiology 41:397–407
    [Google Scholar]
  15. Thouvenot D. R., Bourgeois C. M. 1971; Optimisation de la selection de mutants de Saccharomyces cerevisiae par la nystatine. Annales de Vinstitut Pasteur 120:617–629
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-84-1-111
Loading
/content/journal/micro/10.1099/00221287-84-1-111
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error