@article{mbs:/content/journal/micro/10.1099/00221287-84-1-111, author = "Bal, J. and Balbin, E. and Pieniacźek, N. J.", title = "Method for Isolating Auxotrophic Mutants in Aspergillus nidulans Using N-glycosyl-polifungin", journal= "Microbiology", year = "1974", volume = "84", number = "1", pages = "111-116", doi = "https://doi.org/10.1099/00221287-84-1-111", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-84-1-111", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: A method for the selection of auxotrophic mutants in Aspergillus nidulans using N-glycosyl-polifungin has been developed. The method results in about 9000-fold enrichment. All types of auxotrophs were obtained. Under certain conditions preferential selection of nucleic acid hydrolysate-requiring auxotrophs was observed. The frequency of occurrence of these mutants was about 50% of all colonies tested. Cysteine auxotrophs of A. nidulans were obtained for the first time by means of this method.", }