Synchronous Cultures of Micro-organisms: Large-scale Preparation by Continuous-flow Size Selection
Lloyd, D. and John, Lynda and Edwards, C. and Chagla, A. H.,, 88, 153-158 (1975),
doi = https://doi.org/10.1099/00221287-88-1-153,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= SUMMARY: The separation of the smallest cells from an exponentially growing culture by passage through a continuous flow centrifuge rotor under controlled conditions of rotor speed and flow rate, provides a simple method for the rapid production of large-scale synchronous cultures. The effluent from the rotor, containing the smallest-sized class of organisms, still suspended in their growth medium and still growing undisturbed throughout this rapid procedure, provides a culture which goes on to exhibit synchronous cell division. Successfully applied to budding and fission yeasts and to amoeboid and ciliated protozoa, this procedure is potentially applicable to any non-filamentous, non-aggregating, unicellular organism or to cells of higher plants or animals growing in liquid tissue-cultures.,
language=,
type=