A Plasmid Involved in Chloramphenicol Production in Streptomyces venezuelae: Evidence from Genetic Mapping
Akagawa, H. and Okanishi, M. and Umezawa, H.,, 90, 336-346 (1975),
doi = https://doi.org/10.1099/00221287-90-2-336,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= SUMMARY: To test the hypothesis that chloramphenicol production in Streptomyces venezuelae depends on the presence of a plasmid, mapping analysis was carried out by using eight markers in addition to chloramphenicol production and melanoid pigment formation. The sequence of the eight markers was determined on a circular linkage map as follows: -his-ade-str-leu-lys-met-ilv-pro-(his-). This sequence resulted in the frequency of quadruple crossover (q.c.o.) recombinants having the lowest value, 3·2 to 4·9 %. However, the character of chloramphenicol non-production, which was obtained by incubating mycelia with acriflavin, was not located on this linkage map; more than 15 % q.c.o. recombinants would have been required to explain the results. From these results and other tests, it is concluded that chloramphenicol production is controlled by a plasmid. This plasmid appeared to be non-transferable in conjugation.,
language=,
type=