Vitamin Requirements for Growth of Myxamoebae of Dictyostelium discoideum in a Defined Medium
Watts, D.J.,, 98, 355-361 (1977),
doi = https://doi.org/10.1099/00221287-98-2-355,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= SUMMARY: Myxamoebae of Dictyostelium discoideum strain Ax-2 were adapted to growth in a medium containing B vitamins, a vitamin-free tryptic digest of casein, soluble starch and inorganic salts. Omission of vitamins from this medium confirmed that d-biotin, thiamin, folate and a-lipoate are essential for myxamoebal growth and showed that riboflavin or flavin mononucleotide is also required. No requirement for other vitamins or growth factors could be detected. A defined medium was developed by replacement of the tryptic digest of casein with a mixture of 20 l-amino acids and addition of Tween 80. Tween 80 caused starch precipitation in the medium and this seemed essential for stimulating growth of the myxamoebae.,
language=,
type=