@article{mbs:/content/journal/micro/10.1099/00221287-98-2-587, author = "Masover, Gerald K. and Catlin, John and Hayflick, Leonard", title = "The Effect of Growth and Urea Concentration on Ammonia Production by a Urea-hydrolysing Mycoplasma (Ureaplasma urealyticum)", journal= "Microbiology", year = "1977", volume = "98", number = "2", pages = "587-593", doi = "https://doi.org/10.1099/00221287-98-2-587", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-98-2-587", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: The rate of accumulation of ammonium ion in cultures of Ureaplasma urealyti-cum was independent of the growth rate and of the initial urea concentration above 0·025% in the medium, although the quantity of ammonium ion accumulating did depend on the initial urea concentration. Ammonium ions accumulated at a similar rate in U. urealyticum cultures of both rapidly and slowly growing organisms. Viable but non-growing ureaplasmas also produced ammonia in complete medium at a lower temperature than usual (25°C) or in an inadequate growth medium at 37°C. The rate of ammonium ion accumulation in a dying culture depended on the number of viable organisms present; this is relevant to diagnostic methods for ureaplasmas which depend on detecting ammonia colorimetrically.", }