RT Journal Article SR Electronic(1) A1 Stanley, John A1 Linton, Dennis A1 Burnens, André P. A1 Dewhirst, Floyd E. A1 On, Stephen L. W. A1 Porter, Anthony A1 Owen, Robert J. A1 Costas, MenelaosYR 1994 T1 Helicobacter pullorumsp. nov. - genotype and phenotype of a new species isolated from poultry and from human patients with gastroenteritis JF Microbiology, VO 140 IS 12 SP 3441 OP 3449 DO https://doi.org/10.1099/13500872-140-12-3441 PB Microbiology Society, SN 1465-2080, AB Campylobacter-like organisms were isolated from the liver, duodenum and caecum of broiler and layer chickens, and from humans with gastroenteritis. They formed a unique DNA homology group and a polyphasic taxonomic analysis was made of 16 strains. Analysis of the nucleotide sequence of the 16S rRNA gene from seven of the strains identified them as belonging to a single species, within the genus Helicobacter.This conclusion was supported by the studies of relative DNA homology and of total protein electrophoretic patterns. The new species could be biochemically differentiated from other helicobacters and its ultrastructure in the electron microscope was typical of the genus except that the flagellum was not sheathed. We propose the name Helicobacter pullorumsp. nov. for this group. Like H. fennelliaeor H. cinaediit represents another non-gastric urease-negative Helicobacterspecies colonizing the lower bowel. Its isolation from the livers of chickens with vibrionic hepatitis is significant. We describe a species-specific PCR assay for H. pullorumsp. nov. which will facilitate its identification and further studies of its epidemiology., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-140-12-3441