f Cloning, sequencing and transcriptional studies of the genes for cytochrome c-553 and plastocyanin from Anabaena sp. PCC 7120
- Authors: Majid Ghassemian, Bess Wong, Fernando Ferreira, John L. Markley, Neil A. Straus
- Author for correspondence: Neil A. Straus. Tel: +1 416 978 3532. Fax: +1 416 978 5878. e-mail: firstname.lastname@example.org
- Microbiology, May 1994 140: 1151-1159, doi: 10.1099/13500872-140-5-1151
- Subject: Genetics And Molecular Biology
- Published Online:
In some cyanobacteria and eukaryotic algae, cytochrome c-553 (c-552) and plastocyanin function as alternative electron carriers between the cytochrome b6-f complex and Photosystem I. In these organisms plastocyanin is the electron carrier under copper-replete conditions, and cytochrome c-553 is the electron carrier during copper deprivation. In this paper we report the cloning, sequencing and transcriptional analysis of the genes for cytochrome c-553 and plastocyanin from Anabaena sp. PCC 7120. The gene for cytochrome c-553 encodes a preprotein containing 111 amino acids with a predicted n-terminal transit peptide sequence of 25 amino acids. The gene for plastocyanin encodes a preprotein containing 139 amino acids with a n-terminal transit peptide sequence of 34 amino acids. RNA transcript analyses indicate that the expression of the genes for cytochrome c-553 (petl) and plastocyanin (petE) are regulated in reciprocal ways in response to copper concentration. In copper-replete conditions, petJ is expressed at very low levels, but is transcribed at high levels under copper deprivation; petE is down-regulated in the absence of copper, but is rapidly up-regulated when copper is added back to the medium.
The GenBank accession numbers for the nucleotide sequence data reported in this paper are M97009 and L19417.
- Keyword(s): algae, Anabaena sp. PCC 7120, cytochrome c-553, gene regulation, plastocyanin, cyanobacteria
© Society for General Microbiology 1994 | Published by the Microbiology Society
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