Is mRNA sequestration involved in the regulation of progesterone 14α-hydroxylase cytochrome P-450 expression in Mucor hiemalis?
Antoniou, Tony N. H. and Curran, Brendan P. G. and Smith, Kelvin E.,, 140, 1633-1640 (1994),
doi = https://doi.org/10.1099/13500872-140-7-1633,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= During a 6 h incubation at 26 °C, Mucor hiemalis hydroxylated progesterone predominantly at the 14α site. Further incubation resulted in the production of several 14α-dihydroxyprogesterones and a novel microbial transformation product, 8(9), 14(15)-didehydroprogesterone (pregna-4,8[9], 14[15]-triene-3,20-dione). Azole inhibition indicates that the hydroxylases are cytochromes P-450. Mycelia transformed in the presence of the translation inhibitor cycloheximide failed to hydroxylate progesterone, whereas identical treatment with the transcription inhibitor actinomycin D, or heat-shock for 1 h at 37 °C prior to progesterone transformation, stimulated hydroxylation. 14α-Hydroxylation was stimulated if mycelia were pre-incubated with progesterone, transcription or translation inhibitors. These data are consistent with a model in which cytochrome P-450 steroid hydroxylase mRNA is stored in cells in a dormant form by a labile sequestering protein.,
language=,
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