Isolation and characterization of a strain of Rhodobacter sulfidophilus: A bacterium which grows autotrophically with dimethylsulphide as electron donor
Hanlon, Steven P. and Holt, Robert A. and Moore, Geoffrey R. and McEwan, Alastair G.,, 140, 1953-1958 (1994),
doi = https://doi.org/10.1099/13500872-140-8-1953,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= A marine photosynthetic bacterium (strain SH1) was isolated after enrichment under phototrophic conditions in media containing dimethylsulphide (DMS) and bicarbonate (HCO− 3) as potential carbon sources. Analysis of culture medium using nuclear magnetic resonance spectrometry showed that during phototrophic and chemotrophic growth of strain SH1 on DMS/HCO− 3 dimethylsulphoxide (DMSO) was produced from DMS. These results indicate that strain SH1 grew autotrophically with DMS serving as an electron donor in photosynthesis and respiration, but not as a carbon source. Biochemical characterization and 16S rRNA analysis indicated that the isolate was a strain of Rhodobacter sulfidophilus. An assay for the enzyme catalysing the oxidation of DMS (DMS:acceptor oxidoreductase) was developed by measuring electron transfer from DMS to 2,6-dichlorophenolindophenol (DCPIP). This reaction was dependent on phenazine ethosulphate to mediate electron transfer from DMS:acceptor oxidoreductase to DCPIP. DMS:acceptor oxidoreductase was found to have a periplasmic location in strain SH1 as was a reduced methylviologen: DMSO oxidoreductase activity. Zymogram staining patterns of periplasmic fractions indicated that DMS: acceptor oxidoreductase and DMSO reductase were distinct enzymes. This was confirmed by resolution of the two activities by gel filtration.,
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