f The Rhizobium leguminosarum biovar viciae nodO gene can enable a nodE mutant of Rhizobium leguminosarum biovar trifolii to nodulate vetch
- Authors: A. Economou†, A. E. Davies, A. W. B. Johnston, J. A. Downie
- Author for correspondence: J. A. Downie. Tel: + 44 603 52571. Fax: +44 603 56844.
- First Published Online: 01 September 1994, Microbiology 140: 2341-2347, doi: 10.1099/13500872-140-9-2341
- Subject: Plant-Microbe Interactions
- Issue Published:
Analysis of the nodulation characteristics of transposon-induced mutants of Rhizobium leguminosarum bv. viciae revealed that nodO and the closely-linked rhi genes contribute to nodulation of peas (Pisum sativum) and the vetch Vicia hirsuta. Although mutation of nodO alone had no significant effect on nodulation of either legume, a double mutant lacking both nodO and nodE nodulated both legumes very poorly. Similarly, a double mutant lacking nodE and either rhiA or rhiB nodulated peas less efficiently than a nodE mutant. Thus, although mutations affecting only the rhi genes normally have no observed effect on nodulation, these genes do appear to contribute to pea nodulation. When transferred to a wild-type strain of Rhizobium leguminosarum bv. trifolii, neither nodO nor the rhi gene region conferred pea or vetch nodulating ability. However, in a nodE mutant of R. I. bv. trifolii, nodO did confer a significant level of vetch nodulating ability, indicating that the secreted NodO protein can play a role in determining legume recognition by R. I. bv. viciae.
Present address: Department of Biochemistry, Dartmouth Medical school, 7200 Vail Building, Room 425, Hanover, NH 03755, USA.
© Society for General Microbiology 1994 | Published by the Microbiology Society
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