The Rhizobium leguminosarum biovar viciae nodO gene can enable a nodE mutant of Rhizobium leguminosarum biovar trifolii to nodulate vetch
Economou, A. and Davies, A. E. and Johnston, A. W. B. and Downie, J. A.,, 140, 2341-2347 (1994),
doi = https://doi.org/10.1099/13500872-140-9-2341,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Analysis of the nodulation characteristics of transposon-induced mutants of Rhizobium leguminosarum bv. viciae revealed that nodO and the closely-linked rhi genes contribute to nodulation of peas (Pisum sativum) and the vetch Vicia hirsuta. Although mutation of nodO alone had no significant effect on nodulation of either legume, a double mutant lacking both nodO and nodE nodulated both legumes very poorly. Similarly, a double mutant lacking nodE and either rhiA or rhiB nodulated peas less efficiently than a nodE mutant. Thus, although mutations affecting only the rhi genes normally have no observed effect on nodulation, these genes do appear to contribute to pea nodulation. When transferred to a wild-type strain of Rhizobium leguminosarum bv. trifolii, neither nodO nor the rhi gene region conferred pea or vetch nodulating ability. However, in a nodE mutant of R. I. bv. trifolii, nodO did confer a significant level of vetch nodulating ability, indicating that the secreted NodO protein can play a role in determining legume recognition by R. I. bv. viciae. ,
language=,
type=