1887

Abstract

Summary: Nucleotide sequence analysis of the and loci, required for the synthesis of methanol dehydrogenase in XX, has revealed two open reading frames that show significant similarity to sequences of prokaryotic two-component systems, especially MxaY and MxaX proteins of another methylotrophic bacterium, Cell-free extracts and DNA-column-fractionated proteins from wild-type XX cells grown on methanol or succinate contained protein(s) that were able to bind specifically to the upstream region of methanol dehydrogenase large subunit gene (). In contrast, cell-free extracts from and mutant strains of XX had zero or reduced binding activity towards the promoter fragments of the gene. This is consistent with the involvement of the and genes in transcriptional regulation of methanol dehydrogenase synthesis. Analyses of sequential deletions of the upstream region have defined the functional boundary of the promoter/operator region of this gene and identified one nucleotide segment as essential to the activation of .

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1995-10-01
2024-04-20
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References

  1. Allen L.N., Hanson R.S. 1985; Construction of broad-host- range cosmid cloning vectors: identification of genes necessary for growth of Methylobacterium organophilum XX on methanol.. J Bacteriol 161:955–962
    [Google Scholar]
  2. Anderson D.J., Morris C.J., Nunn D.N., Anthony C., Lidstrom M.E. 1990; Nucleotide sequence of the Methylobacterium extorquens AMI moxJ and moxj genes involved in methanol oxidation.. Gene 90:173–176
    [Google Scholar]
  3. Anthony C. 1982 The Biochemistry of Methylotrophs. New York: Academic Press;
    [Google Scholar]
  4. Anthony C. 1986; Bacterial oxidation of methane and methanol.. Adv Microb Physiol 27:113–210
    [Google Scholar]
  5. Arico B., Miller J., Roy C., Stibitz S., Monack D., Falkow S., Gross R., Rappuoli R. 1989; Sequences required for expression of Bordetella pertussis virulence factors share homology with prokaryotic signal transduction proteins.. Proc Natl Acad Sci USA 866671–6675
    [Google Scholar]
  6. Bastien C, Machlin S., Zhang Y., Donaldson K., Hanson R.S. 1989; Organization of genes required for the oxidation of methanol to formaldehyde in three type II methylotrophs.. Appl Environ Microbiol 55:3124–3130
    [Google Scholar]
  7. Chodosh L.A. 1989; Mobility shift DNA-binding assay using gel electrophoresis.. In Current Protocols in Molecular Biology pp. 12.2.1–12.2.10. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. Edited by New York: John Wiley;
    [Google Scholar]
  8. Cox J.M., Day D.J., Anthony C. 1992; The interaction of methanol dehydrogenase and its electron acceptor, cytochrome cL, in methylotrophic bacteria.. Biochim Biophys Acta 1119:97–106
    [Google Scholar]
  9. Harms N., Reijnders W.N.M., Anazawa H., van der Palen C.J.N.M., van Spanning R.J.M., Oltmann L.F., Stouthamer A.H. 1993; Identification of a two-component regulatory system controlling methanol dehydrogenase synthesis in Paracoccus denitrificans. . Mol Microbiol 8:457–470
    [Google Scholar]
  10. Henner D.J., Yang M., Ferrari E. 1988; Localization of Bacillus subtilis sacU(Hy) mutations to two linked genes with similarities to the conserved procaryotic family of two-component signaling systems.. J Bacteriol 170:5102–5109
    [Google Scholar]
  11. Hess J., Oosawa K., Kaplan N., Simon M.I. 1988; Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis.. Cell 53:79–87
    [Google Scholar]
  12. Igo M.M., Ninfa A.J., Silhavy T.J. 1989; A bacterial environmental sensor that functions as a protein kinase and stimulates transcriptional activation.. Genes & Dev 3:598–605
    [Google Scholar]
  13. Island M.D., Wei B.-Y., Kadner R.J. 1992; Structure and function of the uhp genes for the sugar phosphate transport system in Escherichia coli and Salmonella typhimurium. . J Bacteriol 174:2754–2762
    [Google Scholar]
  14. Kahn D., Ditta G. 1991; Modular structure of FixJ : homology of the transcriptional activator domain with the -35 binding domain of sigma factors.. Mol Microbiol 5:987–997
    [Google Scholar]
  15. Kaminski P.A., Elmerich C. 1991; Involvement offixEJ in the regulation of nitrogen fixation in Azorhizobium caulinodans. . Mol Microbiol 5:665–673
    [Google Scholar]
  16. Lidstrom M.E., Anthony C, Biville F., Gasser F., Goodwin P., Hanson R.S., Harms N. 1994; New unified nomenclature for genes involved in the oxidation of methanol in Gram-negative bacteria.. FEMS Microbiol Lett 117:103–106
    [Google Scholar]
  17. Machlin S.M. 1987 Genetic studies of methanol dehydrogenase synthesis in the facultative methylotroph Methylobacterium organophilum XX. PhD thesis University of Minnesota:
    [Google Scholar]
  18. Machlin S.M., Hanson R.S. 1988; Nucleotide sequence and transcriptional start site of the Methylobacterium organophilum XX methanol dehydrogenase structural gene.. J Bacteriol 170:4739–4747
    [Google Scholar]
  19. Machlin S.M., Tam P.E., Bastien C.A., Hanson R.S. 1988; Genetic and physical analyses of Methylobacterium organophilum XX genes encoding methanol oxidation.. J Bacteriol 170:141–148
    [Google Scholar]
  20. MacLennan D.G., Onsby J.C, Vasey R. B., Cotton N.T. 1971; The influence of dissolved oxygen on Pseudomonas AMI grown on methanol in continuous culture.. J Gen Microbiol 69:395–404
    [Google Scholar]
  21. Nohno T., Noji S., Taniguchi S., Saito T. 1989; The narX and narL genes encoding the nitrate-sensing regulators of Escherichia coli are homologous to a family of prokaryotic two-component regulatory genes.. Nucleic Acids Res 17:2947–2957
    [Google Scholar]
  22. Nunn D.N., Day D., Anthony C. 1989; The second subunit of methanol dehydrogenase of Methylobacterium extorquens AMI.. Biochem J 260:857–862
    [Google Scholar]
  23. O’Connor M.L., Hanson R.S. 1977; Enzyme regulation in Methylobacterium organophilum. . J Gen Microbiol 98:265–272
    [Google Scholar]
  24. Patt T.E., Cole G.C., Hanson R.S. 1976; Methylobacterium, a new genus of facultative methylotrophic bacteria.. Int J Syst Bacteriol 26:226–229
    [Google Scholar]
  25. Rabin R.S., Stewart V. 1992; Either of two functionally redundant sensor proteins, NarX and NarQ, is sufficient for nitrate regulation in Escherichia coli K-12.. Proc Natl Acad Sei USA 898419–8423
    [Google Scholar]
  26. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning-, a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Stock J.B., Ninfa A.J., Stock A.M. 1989; Protein phosphorylation and regulation of adaptive responses in bacteria.. Microbiol Rev 53:450–490
    [Google Scholar]
  28. Stout V., Gottesman S. 1990; RcsB and RcsC: a two- component regulator of capsule synthesis in Escherichia coli. . J Bacteriol 172:659–669
    [Google Scholar]
  29. Tanaka T., Kawata M. 1988; Cloning and characterization of Bacillus subtilis iep, which has positive and negative effects on production of extracellular proteases.. J Bacteriol 170:3593–3600
    [Google Scholar]
  30. de Vries G.E., Kues U., Stahl U. 1990; Physiology and genetics of methylotrophic bacteria.. FEMS Microbiol Rev 75:57–102
    [Google Scholar]
  31. Xu H.H., Viebahn M., Hanson R.S. 1993; Identification of methanol regulated promoter sequences from the facultative methylotrophic bacterium Methylobacterium organophilum XX.. J Gen Microbiol 139:743–752
    [Google Scholar]
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