Glycosylation of the flagellin of the polar flagellum of Azospirillum brasilense, a Gram-negative nitrogen-fixing bacterium Moens, Sara and Michiels, Kris and Vanderleyden, Jos,, 141, 2651-2657 (1995), doi = https://doi.org/10.1099/13500872-141-10-2651, publicationName = Microbiology Society, issn = 1350-0872, abstract= Summary: The glycosylation of the flagellin of the polar flagellum of Azospirillum brasilense Sp7 is demonstrated in several ways: (1) by a decrease in apparent M r after chemical deglycosylation; (2) by sugar staining after SDS-PAGE; (3) by use of a sugar-specific monoclonal antibody in immunogold labelling coupled with transmission electron microscopy. In addition, two mutant flagellins are described; they differ in glycan composition when compared with the wild-type. The glycosylation of a bacterial flagellin is unusual and has to be taken into account in the proposed model for the assembly of the flagellar filament., language=, type=