f Molecular cloning of a Coxiella burnetii gene encoding a macrophage infectivity potentiator (Mip) analogue
- Authors: Yin-Yuan Mo†, Nicholas P. Cianciotto, Louis P. Mallavia3
- Author for correspondence: L. P. Mallavia. Tel: + 1 509 335 3323. Fax: +1 509 335 3517. e-mail: mallavia @ wsu.edu
- First Published Online: 01 November 1995, Microbiology 141: 2861-2871, doi: 10.1099/13500872-141-11-2861
- Subject: Genetics And Molecular Biology
- Issue Published:
Summary: The gene encoding a protein that reacted with antibodies specific for Legionella pneumophila macrophage infectivity potentiator (LpMip) was cloned from Coxiella burnetii, the obligate intracellular rickettsia that causes Q fever in humans. Nucleotide sequencing analysis revealed an ORF encoding a gene product of 230 amino acids with a molecular mass of 25-5 kDa and a predicted pI of 10-7. The predicted amino acid sequence from the ORF shows similarity with Mip/Mip-like proteins of Legionella (46%) and Chlamydia (30%). Moreover, like LpMip, the amino acid sequence of the C terminus of this protein has over 35% identity to prokaryotic and eukaryotic FK506-binding proteins (FKBPs) that belong to a superfamily of immunophilins and are peptidyl-prolyl cis-trans isomerases (PPlases). When overproduced in Escherichia coli, the C. burnetii protein also exhibited PPlase activity. Taken together, these results demonstrate that C. burnetii encodes a Mip analogue (CbMip). A putative leader peptide at the N terminus of CbMip was detected by computer analysis. Furthermore, TnphoA mutagenesis demonstrated that in E. coli CbMip was secreted. In view of the role of Mip/Mip-like proteins in the pathogenesis of Legionella and Chlamydia, CbMip may be a C. burnetii virulence factor.
Present address: Department of Tumor Cell Biology, St Jude Children's Research Hospital, 322 North Lauderdale, Memphis, TN 38101, USA.
© Society for General Microbiology 1995 | Published by the Microbiology Society
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