1887

Abstract

SUMMARY:

The gene encoding extracellular xylanase () was amplified as a 770 bp DNA fragment from 168 chromosomal DNA by PCR. The genes encoding endo-β-1,4-glucanase () and endo-β-1,3-1,4-glucanase () were isolated from a genomic library of 168. The sequences of and were identical to those of the xylanase and cellulase genes from PAP115. Integrative plasmids containing DNA fragments with deletions in the coding region of the genes were constructed and used to replace the chromosomal and genes of 168. Strains without any detectable activity against xylan (Xyn), carboxymethylcellulose (Egl) or mixed linked β-1,3-1,4-glucan (Egl Bgl) were obtained. The genes were mapped at 170° 175° and 340° on thechromosome.

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1995-02-01
2024-03-28
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References

  1. Anagnostopoulos C., Piggot P. J., Hoch A. J. 1993 The genetic map of Bacillus subtilis . In Bacillus subtilis and Other GramPositive Bacteria: Biochemistry, Physiology and Molecular Genetics pp 425–461 Edited by Sonenshein A. L., Hoch J. A., Losick R. Washington DC: American Society for Microbiology;
    [Google Scholar]
  2. Anderson M. A., Stone B. A. 1975; A new substrate for investigating the specificity of β-glucan hydrolases. FEBS Lett 52 202–207
    [Google Scholar]
  3. Azevedo V., Alvarez E., Zumstein E., Damiani G., Sgaramella V., Ehrlich S. D., Serror P. 1993; An ordered collection of Bacillus subtilis DNA segments cloned in yeast artificial chromosomes. Proc Natl Acad Sci USA 90 6047–6051
    [Google Scholar]
  4. Bolivar F., Rodriguez R., Green P. J., Betlach M., Heyneker H. L., Boyer H. W., Crosa J., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  5. Borriss R., Süss K. H., Süss M., Manteuffel R., Hofemeister J. 1986; Mapping and properties of bgl (β-glucanase) mutants of Bacillus subtilis . J Gen Microbiol 132:431–442
    [Google Scholar]
  6. Cornelis P., Digneffe C., Willemot K. 1982; Cloning and expression of a Bacillus coagulans amylase gene in Escherichia coli . Mol & Gen Genet 186:507–511
    [Google Scholar]
  7. Dedonder R. A., Lepesant J., Lepesant-Kejzlarova J., Billault A., Steinmetz M., Kunst F. 1977; Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Appl Environ Microbiol 33:989–993
    [Google Scholar]
  8. Haima P., van Sinderen D., Bron S., Venema G. 1990; An improved β-galactosidase α-complementation system for molecular cloning in Bacillus subtilis . Gene 93:41–47
    [Google Scholar]
  9. Hastrup S. 1988 Analysis of the Bacillus subtilis xylose regulon. In Genetics and Biotechnology of Bacilli pp 79–83 Edited by Ganesan A. T., Hoch J. A. San Diego: Academic Press;
    [Google Scholar]
  10. Henrissat B. 1991; A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 280:309–316
    [Google Scholar]
  11. Henrissat B., Bairoch A. 1993; New families in the classification of glycosyl hydrolases based on amino acid sequence similarities.. Biochem J 293:781–788
    [Google Scholar]
  12. Kawamura F., Doi R. H. 1984; Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases. J Bacteriol 160:442–444
    [Google Scholar]
  13. Krüger St., Stülke J., Hecker M. 1993; Catabolite repression of β-glucanase synthesis in Bacillus subtilis . J Gen Microbiol 139:2047–2054
    [Google Scholar]
  14. Lederberg E. M., Cohen S. N. 1974; Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol 119:1072–1074
    [Google Scholar]
  15. Lindner C, Stülke J., Krüger St., Hecker M. 1993 Induktion der β-Glucanase-Synthese in Bacillus subtilis V.27. In Proceedings Erwin Kiesch Symposium iiber Plasmide und Genregulation Sellin, Rügen; Germany:
    [Google Scholar]
  16. Lindner C, Stülke J., Hecker M. 1994; Regulation of xylanolytic enzymes in Bacillus subtilis . Microbiology 140:753–757
    [Google Scholar]
  17. Lo A. C., MacKay R. M., Seligy V. L., Willick G. C. 1988; Bacillus subtilis β -1,4-endoglucanase products from intact and truncated genes are secreted into the extracellular medium by Escherichia coli . Appl Environ Microbiol 54:2287–2292
    [Google Scholar]
  18. MacKay R. M., Lo A., Willick G., Zuker M., Baird S., Dove M., Moranelli F., Seligy V. 1986; Structure of a Bacillus subiilis endo-β-l,4-glucanase gene. Nucleic Acids Res 14:9159–9170
    [Google Scholar]
  19. Mead D. A., Szczwsna-Skorupa E., Kemper B. 1986; Singlestranded DNA ‘blue’ T7-promoter plasmids: a versatile tandem promoter system for cloning and protein engineering. Protein Eng 1:67–74
    [Google Scholar]
  20. Michel B., Niaudet B., Ehrlich S. D. 1983; Intermolecular recombination during transformation of Bacillus subtilis competent cells by monomeric and chimeric plasmids.. Plasmid 10:1–10
    [Google Scholar]
  21. Murphy N., McConnell D. J. M., Cantwell B. A. 1984; The DNA sequence of the gene and genetic control sites for the excreted Bacillus subtilis enzyme β-glucanase.. Nucleic Acids Res 12:5355–5367
    [Google Scholar]
  22. O'Kane C., Cantwell B. A., McConnell D. J. M. 1985; Mapping of the gene for endo-β-1,3-1,4-glucanase of Bacillus subtilis . FEMS Microbiol Lett 29:135–139
    [Google Scholar]
  23. Olsen O., Borriss R., Simon O., Thomsen K. K. 1991; Hybrid Bacillus (1-3,1-4)-β-glucanases: engineering thermostable enzymes by construction of hybrid genes. Mol & Gen Genet 225:177–185
    [Google Scholar]
  24. Paice M. G., Bourbonnais R., Desrochers M., Jurasek L., Yaguchi M. 1986; A xylanase gene from Bacillus subtilis: nucleotide sequence and comparison with B. pumilus gene. Arch Microbiol 144:201–206
    [Google Scholar]
  25. Putzer H. A., Brakhage A. A., Grunberg-Manago M. A. 1990; Independent genes for two threonyl-tRNA synthetases in Bacillus subtilis . J Bacteriol 172:4593–4602
    [Google Scholar]
  26. Roncero M. I. 1983; Genes controlling xylan utilization by Bacillus subtilis . J Bacteriol 156:257–263
    [Google Scholar]
  27. Saito H., Miura K. I. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629
    [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  29. Shima S., Igarashi Y., Kodama T. 1993; Purification and properties of two truncated endoglucanases produced in Escherichia coli harbouring Clostridium cellulolyticum endoglucanase gene celCCD . Appl Microbiol Biotechnol 38:750–754
    [Google Scholar]
  30. Stahl M. L., Ferrari E. 1984; Replacement of the Bacillus subtilis subtilisin structural gene with an in vitro-derived deletion mutation. J Bacteriol 158:411–418
    [Google Scholar]
  31. Takahashi M., Hashimoto Y. 1963; Studies on bacterial xylanase. IV. On the nature of the crystalline xylanase of Bacillus subtilis G2.. Hakko Kogaku Zasshi 41:186–189
    [Google Scholar]
  32. Tam Ng. H., Borriss R. 1995; The thy A gene from Bacillus subtilis exhibits extensive similarity with the phage ϕ 3T thymidylate synthase gene. Microbiology 141:291–297
    [Google Scholar]
  33. Thomson J. A. 1993; Molecular biology of xylan degradation. FEM Microbiol Rev 104:65–82
    [Google Scholar]
  34. Vandeyar M. A., Zahler S. A. 1986; Chromosomal insertions of Tn917in Bacillus subtilis . J Bacteriol 167:530–534
    [Google Scholar]
  35. Yuuki T., Tezuka H., Yabuchi S. 1989; Purification and some properties of two enzymes from a β-glucanase hyperproducing strain, Bacillus subtilis HL-25. Agric Biol Chem 53:2341–2346
    [Google Scholar]
  36. Zhang Y., Aronson A. 1994; A Bacillus subtilis bglA gene encoding phospho-β-glucosidase is inducible and closely linked to a NADH dehydrogenase-encoding gene. Gene 140:85–90
    [Google Scholar]
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