f Identification of a 60 kb region of the chromosome of Pseudomonas fluorescens NCIB 10586 required for the biosynthesis of pseudomonic acid (mupirocin)
- Authors: Carl A. Whatling‡, John E. Hodgson, Martin K. R. Burnham, Neil J. Clarke†, F. Christopher H. Franklin, Christopher M. Thomas3
- Author for correspondence: Christopher M. Thomas. Tel: + 44 121 414 5903. Fax: +44 121 414 5925.
- First Published Online: 01 April 1995, Microbiology 141: 973-982, doi: 10.1099/13500872-141-4-973
- Subject: Genetics And Molecular Biology
- Issue Published:
Pseudomonic acid (mupirocin) produced by Pseudomonas fluorescens is a polyketide antibiotic which blocks isoleucyl-tRNA synthetase. Knowledge of the biosynthetic pathways leading to pseudomonic acid production may help in engineering related antibiotics with other useful properties. To help define these pathways, we have isolated 13 non-producing mutants using Tn5 and Tn1725 mutagenesis. Seven of the Tn5 insertions mapped within a 55 kb region. The remaining insertions, and one gene encoding resistance to pseudomonic acid, mapped at separate locations. DNA that overlapped the seven clustered Tn5 insertions was isolated on a series of clones, extending over a region in excess of 60 kb. Non-producing mutants generated via gene disruption suggest that the biosynthetic cluster extends throughout this region.
Present address: Department of Genetics, John Innes Institute, Colney Lane, Norwich NR4 7UH, UK.
Present address: Glaxo D & R Ltd, Greenford Road, Greenford, Middlesex UB6 OHE, UK
© Society for General Microbiology 1995 | Published by the Microbiology Society
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