f The bceT gene of Bacillus cereus encodes an enterotoxic protein
- Authors: Norio Agata3, Michio Ohta, Yoshichika Arakawa, Masashi Mori
- Author for correspondence: Norio Agata. Tel: +81 52 841 1511, ext. 51. Fax: +81 52 841 1514.
- First Published Online: 01 April 1995, Microbiology 141: 983-988, doi: 10.1099/13500872-141-4-983
- Subject: Pathogenicity And Medical Microbiology
- Issue Published:
A toxin gene (bceT) on a 2·9 kb DNA fragment of Bacillus cereus B-4ac was cloned and expressed in Escherichia coli, and its nucleotide sequence determined. The DNA fragment contained an open reading frame capable of encoding a polypeptide of 336 amino acids with a molecular mass of 41039 Da. The translated product in E. coli exhibited Vero cell cytotoxicity, and was positive in a vascular permeability assay. It also caused fluid accumulation in a ligated mouse ileal loop and was lethal to mice upon injection. These biological activities are considered characteristic of diarrhoeal enterotoxins. We therefore conclude that this gene, designated bceT, encodes one of the enterotoxic proteins of B. cereus which cause food-borne diarrhoea.
The GenBank accession number for the nucleotide sequence reported in this paper isD17312
© Society for General Microbiology 1995 | Published by the Microbiology Society
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