1887

Abstract

The control region of the mannitol ) operon of has been shown to contain five cAMP receptor protein (CRP) binding sequences, the most yet reported for any operon. A DNA fragment encompassing the entire operon regulatory region was generated by PCR, and the binding of the cAMP-CRP complex was studied. Using restrictional analysis to separate, delineate and destroy the various putative CRP binding sites, all five sites were shown to be functional for CRP binding . Four of these sites bound the cAMP-CRP complex with high affinity while the fifth site (the most distal relative to the transcriptional start site) bound the complex with lower affinity. Simultaneous binding of cAMP-CRP complexes to several of these sites was demonstrated. The results serve to identify and define five dissimilar CRP binding sites in a single operon of . A model for operon transcriptional initiation and repression complexes is presented.

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1995-08-01
2024-04-20
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References

  1. Barber A.M., Zhurkin V.B. 1990; CAP binding sites reveal pyrimidine-purine pattern characteristic of DNA bending. J Biomol Struct & Dynam 8:213–232
    [Google Scholar]
  2. Botsford J.L., Harman J.G. 1992; Cyclic AMP in prokaryotes. Microbiol Rev 56:100–122
    [Google Scholar]
  3. Busby S., West D., Lawes M., Webster C., Ishihama A., Kolb A. 1994; Transcription activation by the Escherichia coli cyclic AMP receptor protein. Receptors bound in tandem at promoters can interact synergistically. J Mol Biol 241:341–352
    [Google Scholar]
  4. Chin A.M., Feldheim D.A., Saier M.H. Jr 1989; Altered transcriptional patterns affecting several metabolic pathways in strains of Salmonella typhimurium which overexpress the fructose regulon. J Bacteriol 171:2424–2434
    [Google Scholar]
  5. Davis T., Yamada M., Elgort M., Saier M.H. Jr 1988; Nucleotide sequence of the mannitol {mtl) operon in Escherichia coli. . Mol Microbiol 2:405–412
    [Google Scholar]
  6. Ebright R. 1993; Transcription activation at class I CAP- dependent promoters. Mol Microbiol 8:797–802
    [Google Scholar]
  7. Figge R.M., Ramseier T.M., Saier M.H. Jr 1994; The mannitol repressor (MtlR) of Escherichia coli. . J Bacteriol 176:840–847
    [Google Scholar]
  8. Fox D.K., Presper K.A., Adhya S., Roseman S., Garges S. 1992; Evidence for two promoters upstream of the pts operon: regulation by the cAMP receptor protein regulatory complex. Proc Natl Acad Sci USA 89:7056–7059
    [Google Scholar]
  9. Gerlach P., SØgaard-Andersen L, Pedersen H., Martinussen J., Valentin-Hansen P., Bremer E. 1991; The cyclic AMP (cAMP)-cAMP receptor protein complex functions both as an activator and as a corepressor at the tsx-p2 promoter of Escherichia coli K-12. J Bacteriol 173:5419–5430
    [Google Scholar]
  10. Hanamura A., Aiba H. 1992; A new aspect of transcriptional control of the Escherichia coli crp gene: positive autoregulation. Mol Microbiol 6:2489–2497
    [Google Scholar]
  11. Ishihama A. 1993; Protein-protein communication within the transcription apparatus. J Bacteriol 175:2483–2489
    [Google Scholar]
  12. Jiang W., Wu L.-F., Tomich J., Saier M.H. Jr Niehaus W.G. 1990; Corrected sequence of the mannitol (mtl) operon in Escherichia coli. . Mol Microbiol 4:2003–2006
    [Google Scholar]
  13. Joung J.K., Le L.U., Hochschild A. 1993; Synergistic activation of transcription by Escherichia coli cAMP receptor protein. Proc Natl Acad Sci USA 90:3083–3087
    [Google Scholar]
  14. Kolb A., Busby S., Buc H., Garges S., Adhya S. 1993a; Transcriptional regulation by cyclic AMP and its receptor protein. Anna Rev Biochem 62:749–795
    [Google Scholar]
  15. Kolb A., Igarashi K., Ishihama A., Lavigne M., Buckle M., Buc H. 1993b; E. coli RNA polymerase, deleted in the C-terminal part of its ɑ-subunit, interacts differently with the cAMP-CRP complex at the lacPI and galP 1 promoter. Nucleic Acids Res 21:319–326
    [Google Scholar]
  16. Kumar A., Grimes B., Fujita N., Makino K., Malloch R., Hayward R., Ishihama A. 1994; Role of the sigma 70 subunit of Escherichia coli RNA polymerase in transcription activation. J Mol Biol 235:405–413
    [Google Scholar]
  17. Lee GA., Saier M.H. JR 1983; Mannitol-specific Enzyme II of the bacterial phospho-transferase system. III. The nucleotide sequence of the permease gene. J Biol Chem 258:10761–10767
    [Google Scholar]
  18. Magasanik B., Neidhardt F.C. 1987; Regulation of carbon and nitrogen utilization. In Escherichia coli and Salmonella typhimurium. Cellular and Molecular Biology pp. 1318–1325 Neidhardt J.C., Ingraham J.L., Magasanik B., Low K.B., Schaechter M., Umbarger H.E. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  19. Pedersen H., SØgaard-Andersen L, Holst B., Valentin-Hansen P. 1991; Heterologous cooperativity in Escherichia coli. The CytR repressor both contacts DNA and the cAMP receptor protein when binding to the deoP2 promoter. J Biol Chem 266:17804–17808
    [Google Scholar]
  20. Pérez-Martín J., Rojo F., deLorenzo V. 1994; Promoters responsive to DNA bending : a common theme in prokaryotic gene expression. Microbiol Rev 58:268–290
    [Google Scholar]
  21. Ramseier T.M., Nègre D., Cortay J.-G, Scarabel M., Cozzone A.J., Saier M.H. Jr 1993; In vitro binding of the pleiotropic transcriptional regulatory protein, FruR, to the fru,pps,pts, icd and ace oprons of Escherichia coli and Salmonella typhimurium. . J Mol Biol 234:28–44
    [Google Scholar]
  22. Ramseier T.M., Bledig S., Michotey V., Feghali R., Saier M.H. Jr 1995; The global regulatory protein, FruR, modulates the direction of carbon flow in Escherichia coli. . Mol Microbiol in press
    [Google Scholar]
  23. Richet E., SØgaard-Andersen L. 1994; CRP induces the repositioning of MalT at the Escherichia coli malKp promoter primarily through DNA bending. EMBO J 13:4558–4567
    [Google Scholar]
  24. Saier M.H. Jr 1991; A multiplicity of potential carbon catabolite repression mechanisms in prokaryotic and eukaryotic microorganisms. New Biol 3:1137–1147
    [Google Scholar]
  25. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning, a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Schultz S.G, Shields G.C., Steitz T.A. 1991; Crystal structure of a CAP-DNA complex: the DNA is bent by 90°. Science 253:1001–1007
    [Google Scholar]
  27. West D., Williams R., Rhodius V., Bell A., Sharma N., Zou G, Ishihama A., Busby S. 1993; Interactions between the Escherichia coli cyclic AMP receptor protein and RNA polymerase at class II promoters. Mol Microbiol 10:789–797
    [Google Scholar]
  28. Wu Y., Patil R.V., Datta P. 1992; Catabolite gene activator protein and integration host factor act in concert to regulate tdc operon expression in Escherichia coli. . J Bacteriol 174:6918–6927
    [Google Scholar]
  29. Yamada M., Saier M.H. Jr 1987; Glucitol-specific enzymes of the phosphotransferase system in Escherichia coli. Nucleotide sequence of the gut operon. J Biol Chem 262:5455–5463
    [Google Scholar]
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