f The dnaB-pheA (256°-240°) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism
- Authors: Anil Wipat, Noel Carter, S. Caroline Brignell, B. Jane Guy, Karen Piper, June Sanders, Peter T. Emmerson, Colin R. Harwood3
- 3Author for correspondence: Colin Harwood. Tel: +44 191 222 7708. Fax: +44 191 222 7736. e-mail: email@example.com
- Microbiology, November 1996 142: 3067-3078, doi: 10.1099/13500872-142-11-3067
- Subject: Bacillus Subtilis Genome Sequencing Project
- Published Online:
Within the framework of the international programme to sequence the genome of Bacillus subtilis strain 168, we were allocated the region between dnaB (256°) and pheA (240°). The sequencing of this region is now complete and we report our primary analysis of the 114 kb region containing 114 ORFs. In addition to previously characterized genes, we have identified genes involved in the utilization of plant cell wall polysaccharides, stress responses and the metabolism of amino acids, cell walls, DNA and fatty acids. We also discuss various structural and physical features, including the orientation of genes with respect to replication, putative start and stop codons, ribosome binding sites and ρ-independent transcription terminators.
- Keyword(s): stress genes, genome sequencing, Bacillus subtilis, Long Range PCR, polysaccharide utilization
© Society for General Microbiology 1996 | Published by the Microbiology Society
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