1887

Abstract

Following a recent phylogenetic analysis of all 39 species of the genus and related organisms, the name has been proposed for bacteria previously considered either as or as -like amoebal pathogens’ (LLAPs). To investigate the phylogenetic integrity of this newly proposed species, we determined the 16S rRNA gene sequences of 10 LLAPs isolated from various environmental sources. All 10 isolates clustered within a monophyletic group containing all other members of the genus Eight of the 10 isolates formed a monophyletic subgroup within the genus which also included the two previously characterized strains. Four of these 10 isolates shared a specific and very close relationship with the type strain (> 99% sequence similarity). However, although clearly legionellae, the remaining two LLAP strains bore no specific evolutionary relationship to either or any other species (<96% sequence similarity). Both isolates lay on their own relatively deep-rooted branches within the radiation of the cluster. LLAPs do not therefore, represent a unique species or even a single line of descent within the genus, and investigation of more isolates may reveal them to be as evolutionarily diverse as the other presently recognized species.

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1996-12-01
2024-03-29
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References

  1. Barbaree J. M., Fields B. S., Feeley J. C., Gorman G. W., Martin W. T. 1986; Isolation of protozoa from water associated with a legionellosis outbreak and demonstration of intracellular multiplication of Legionella pneumophila. Appl Environ Microbiol 51:422–424
    [Google Scholar]
  2. Benson R. F., Drozanski W. J., Rowbotham T. J., Bialkowska I., Losos D., Butler J. C., Lipman H. B., Plouffe J. F., Fields B. S. 1995; Serologic evidence of infection with 9 Legionella- like amoebal pathogens in pneumonia patients. 95th ASM General Meeting, Washington DC, USA, 21-25 May 1995 Abstract C-200 p. 35:
    [Google Scholar]
  3. Birtles R. J., Harrison T. G., Fry N. K., Saunders N. A., Taylor A. G. 1991; Taxonomic considerations of Bartonella bacilliformisbased on phylogenetic and phenotypic characteristics. FEMS Microbiol Lett 83:187–192
    [Google Scholar]
  4. Dessen P., Fondrat C., Valencien C., Mugnier C. 1990; BISANCE: a French service for access to biomolecular sequence databases. Comput Appl Biosci 6:355–356
    [Google Scholar]
  5. Drozanski W. J. 1991; Sarcobium lyticum gen. nov., sp. nov., an obligate intracellular bacterial parasite of small free-living amoebae. Int J Syst Bacteriol 41:82–87
    [Google Scholar]
  6. Fallon R. J., Rowbotham T. J. 1990; Microbiological investigations into an outbreak of Pontiac fever due to Legionella micdadeiassociated with use of a whirlpool. J Clin Pathol 43:479–483
    [Google Scholar]
  7. Fields B. S., Shotts E. B., Feeley J. C., Gorman G. W., Martin W. T. 1984; Proliferation of Legionella pneumophila as an intracellular parasite of the ciliated protozoan Tetrahymena pyriformis. In Legionella: Proceedings of the 2nd International Symposium pp. 327–328 Thornsberry C., Balows A., Feeley J. C., Jakubowski W. Edited by Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  8. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees. A method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284
    [Google Scholar]
  9. Fry N. K., Rowbotham T. J., Saunders N. A., Embley T. M. 1991a; Direct amplification and sequencing of the 16S rDNA of an intracellular Legionella species recovered by amoebal enrichment from the sputum of a patient with pneumonia. FEMS Microbiol Lett 83:165–168
    [Google Scholar]
  10. Fry N. K., Warwick S., Saunders N. A., Embley T. M. 1991b; The use of 16S ribosomal RNA analyses to investigate the phylogeny of the family Legionellaceae. J Gen Microbiol 137:1215–1222
    [Google Scholar]
  11. Giles B. L., Fields B. S., Newsome A. L., Drozanski W. J. 1995; Cultivation of Sarcobium lyticum on artificial medium. 95th ASM General Meeting, Washington DC, USA, 21-25 May 1995 Abstract Q-447 p. 478:
    [Google Scholar]
  12. Harrison T. G., Taylor A. G. 1988; The diagnosis of legionnaires̓ disease by estimation of specific antibody levels. In A Laboratory Manual for Eegionella pp. 113–135 Harrison T. G., Taylor A. G. Edited by Chichester:: John Wiley.;
    [Google Scholar]
  13. Higgins D., Bleasby A., Fuchs R. 1992; CLUSTAL v: improved software for multiple sequence analysis. Comput Appl Biosci 8:188–192
    [Google Scholar]
  14. Hookey J. V., Saunders N. A., Fry N. K., Birtles R. J., Harrison T. G. 1996; Phylogeny of Legionellaceae based on small-subunit ribosomal DNA sequences and proposal of Legionella lytica comb, nov. for Legionella- like amoebal pathogens. Int J Syst Bacteriol 46:526–531
    [Google Scholar]
  15. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp. 21–132 Munro N. H. Edited by New York:: Academic Press.;
    [Google Scholar]
  16. McDade J. E., Shepard C. C., Fraser D. W., Tsai T. R., Redus M. A., Dowdle W. R. the laboratory investigation team 1977; Legionnaires̓ disease: isolation of a bacterium and demonstration of its role in other respiratory disease. N Engl J Med 297:1197–1203
    [Google Scholar]
  17. Rowbotham T. J. 1980; Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae. J Clin Pathol 33:1179–1183
    [Google Scholar]
  18. Rowbotham T. J. 1983; Isolation of Legionella pneumophila from clinical specimens via amoebae, and the interaction of those and other isolates with amoebae. J Clin Pathol 36:978–986
    [Google Scholar]
  19. Rowbotham T. J. 1993; Legionella- like amoebal pathogens. In Legionella, Current Status and Emerging Perspectives pp. 137–140 Barbaree J. M., Breiman R. F., Dufour A. P. Edited by Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  20. Springer N., Ludwig W., Drozanski W., Amann R., Schleifer K. H. 1992; The phylogenetic status of Sarcobium lytica, an obligate intracellular parasite of small amoebae. FEMS Microbiol Lett 96:199–202
    [Google Scholar]
  21. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA:DNA reassociation and 16S rDNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
    [Google Scholar]
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