f Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli
- Authors: Jonathan Reizer, Tom M. Ramseier, Aiala Reizer, Alain Charbit, Milton H. Saier jr
- Author for correspondence: Milton H. Saier, Jr. Tel: +1619 534 4084. Fax: +1619 534 7108. e-mail: email@example.com
- Microbiology, February 1996 142: 231-250, doi: 10.1099/13500872-142-2-231
- Subject: Biochemistry
- Published Online:
Summary: We have analysed a gene cluster in the 67.4-76.0 min region of the Escherichia coli chromosome, revealed by recent systematic genome sequencing. The genes within this cluster include: (1) five genes encoding homologues of the E. coli mannose permease of the phosphotransferase system (IIB, IIB', IIC, IIC' and IID); (2) genes encoding a putative N-acetylgalactosamine 6-phosphate metabolic pathway including (a) a deacetylase, (b) an isomerizing deaminase, (c) a putative carbohydrate kinase, and (d) an aldolase; and (3) a transcriptional regulatory protein homologous to members of the DeoR family. Evidence is presented suggesting that the aldolase-encoding gene within this cluster is the previously designated kba gene that encodes tagatose-1,6-bisphosphate aldolase. These proteins and a novel IIAMan-like protein encoded in the 2.4-4.1 min region are characterized with respect to their sequence similarities and phylogenetic relationships with other homologous proteins. A pathway for the metabolism of N-acetylgalactosamine biochemically similar to that for the metabolism of N-acetylglucosamine is proposed.
- Keyword(s): N-acetylgalacrosamine, Escherichia coligenome, aldolases, phosphotransferase system, sugar transport
© Society for General Microbiology 1996 | Published by the Microbiology Society
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