RT Journal Article SR Electronic(1) A1 Reizer, Jonathan A1 Ramseier, Tom M. A1 Reizer, Aiala A1 Charbit, Alain A1 Saier, Milton H.YR 1996 T1 Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli JF Microbiology, VO 142 IS 2 SP 231 OP 250 DO https://doi.org/10.1099/13500872-142-2-231 PB Microbiology Society, SN 1465-2080, AB Summary: We have analysed a gene cluster in the 67.4-76.0 min region of the Escherichia coli chromosome, revealed by recent systematic genome sequencing. The genes within this cluster include: (1) five genes encoding homologues of the E. coli mannose permease of the phosphotransferase system (IIB, IIB', IIC, IIC' and IID); (2) genes encoding a putative N-acetylgalactosamine 6-phosphate metabolic pathway including (a) a deacetylase, (b) an isomerizing deaminase, (c) a putative carbohydrate kinase, and (d) an aldolase; and (3) a transcriptional regulatory protein homologous to members of the DeoR family. Evidence is presented suggesting that the aldolase-encoding gene within this cluster is the previously designated kba gene that encodes tagatose-1,6-bisphosphate aldolase. These proteins and a novel IIAMan-like protein encoded in the 2.4-4.1 min region are characterized with respect to their sequence similarities and phylogenetic relationships with other homologous proteins. A pathway for the metabolism of N-acetylgalactosamine biochemically similar to that for the metabolism of N-acetylglucosamine is proposed., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-142-2-231