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Volume 142, Issue 2

Two chitin synthase genes from Free

Abstract

Summary: PCR was used to amplify fragments corresponding to genes from utilizing as primers oligonucleotides devised according to the conserved regions of fungal genes. The PCR product was employed as a probe to screen a genomic library of the fungus. Two different genes and were thus identified in the positive clones recovered. Their sequence revealed high similarity with the genes previously cloned from other fungi, especially in their central region. Alignment with the deduced protein sequences of all genes reported up to date showed the existence of seven conserved domains. Transcripts from both genes were detected in the yeast and mycelial forms. In general, the transcripts from the gene appeared to be present at a higher level than the transcripts from the gene; the transcripts from both genes appeared to be more abundant in the mycelial form. Gene replacement of either gene and analysis of the resulting phenotype demonstrated that they are non-essential. Nevertheless, growth, chitin synthase activity levels, and chitin content of mycelial cells induced by cultivation in acidic media were all reduced in and mutants. However, mating, virulence and dimorphic behaviour were unaffected. Overall, the results indicate that the and genes encode products with redundant functions in

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Keyword(s): cell wall , chitin synthases , gene replacement and Ustilago maydis
© Society for General Microbiology 1996
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1996-02-01
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Two chitin synthase genes from Ustilago maydis
Microbiology 142, 377 (1996); https://doi.org/10.1099/13500872-142-2-377
/content/journal/micro/10.1099/13500872-142-2-377
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