RT Journal Article SR Electronic(1) A1 Champomier-Vergès, Marie-Christine A1 Stintzi, Alain A1 Meyer, Jean-MarieYR 1996 T1 Acquisition of iron by the non-siderophore-producing Pseudomonas fragi JF Microbiology, VO 142 IS 5 SP 1191 OP 1199 DO https://doi.org/10.1099/13500872-142-5-1191 PB Microbiology Society, SN 1465-2080, AB The iron requirement, siderophore production and iron uptake mechanisms of the type strain Pseudomonas fragi ATCC 4973 and five P. fragi isolates from meat were analysed. The strains exhibited a high sensitivity to iron starvation: their growth was strongly inhibited in medium supplemented with the iron chelator ethylenediamine di(hydroxyphenylacetic acid) or in medium treated with 8-hydroxyquinoline to remove contaminating iron. No siderophores were detectable in the growth supernatants of iron-starved cells. Cross-feeding experiments in iron-depleted medium showed, however, that the bacterial growth could be strongly stimulated by siderophores of foreign origin including desferriferrioxamine B, enterobactin and some pyoverdines. Moreover, all the strains were capable of efficiently using the iron sources present in their natural environment, i.e. transferrin, lactoferrin and haemoglobin. Iron starvation led to the specific production of supplementary outer-membrane proteins of apparent molecular mass ranging from 80 to 88 kDa. Furthermore, growth in the presence of exogenous siderophores resulted, in some strains, in the induction of siderophore-mediated iron uptake systems. For one strain the concomitant synthesis of an iron-regulated, siderophore-inducible outer-membrane protein was observed., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-142-5-1191