1887

Abstract

A strain of was selected for studies on fructose and glucose transport to determine the basis of the fructophilic behaviour of this species. Fructose was transported by a specific low-affinity, high-capacity transport system with a of 65.6 mM and a of 6.7 mmol g h for cells grown on 2% (w/v) fructose, while the transport of glucose showed a of 7 mM and a of 1.7 mmol g h for cells grown on 2% (w/v) glucose. The transporter of glucose also fructose as a substrate. Fructose inactivated the glucose transporter; inactivation was faster at higher concentrations. Both transporters were partially inductive. Measurements of metabolic fluxes and respiration and fermentation rates supported the general features identified by transport measurements. The kinetics and regulation of transport of the two sugars confirm the fructophilic behaviour previously described by other authors.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-7-1733
1996-07-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/7/mic-142-7-1733.html?itemId=/content/journal/micro/10.1099/13500872-142-7-1733&mimeType=html&fmt=ahah

References

  1. Benito B., Lagunas R. 1992; The low-affinity component of Saccharomyces cerevisiae maltose transport is an artifact. J Bacteriol 174:3065–3069
    [Google Scholar]
  2. Bourquelot E. 1886; Recherches sur la fermentation alcoolique d'un mélange de deux sucres.. Ann Chim Phys 9:245–275
    [Google Scholar]
  3. Cirillo V.P. 1968; Relationship between sugar structure and competition for the sugar transport system in baker's yeast. J Bacteriol 95:603–611
    [Google Scholar]
  4. Emmerich W., Radler F. 1983; The anaerobic metabolism of glucose and fructose by Saccharomyces bailii. J Gen Microbiol 129:3311–3318
    [Google Scholar]
  5. Fuhrmann G. F., Storch D., Bode H.-P., Völker B. 1992; Inhibition of glucose transport in Saccharomyces cerevisiae by uranyl ions.. J biotechnol 27:75–84
    [Google Scholar]
  6. Heredia C. F., Sols A., Déla Fuente G. 1968; Specificity of the glucose transport in yeast. Eur J Biochem 5:321–329
    [Google Scholar]
  7. Kotyk A. 1967; Properties of the sugar carrier in baker’s yeast.. Folia Microbiol 12:121–131
    [Google Scholar]
  8. Loureiro-Dias M.C. 1988; Movements of protons coupled to glucose transport in yeasts. A comparative study among 248 yeast strains. Antonie Leeuwenhoek 54:331–343
    [Google Scholar]
  9. Neves L., Pampulha M. E., Loureiro V., Loureiro-Dias M.C. 1994; Resistance of food spoilage yeasts to sorbic acid.. Lett Appl Microbiol 19:8–11
    [Google Scholar]
  10. Peinado J. M., Barbero A., van Uden N. 1987; Repression and inactivation by glucose of the maltose transport system of Candida utilis.. Appl Microbiol biotechnol 26:154–157
    [Google Scholar]
  11. Reifenberger E., Freidel K., Ciriacy M. 1995; Identification of novel HXT genes in Saccharomyces cerevisiae reveals the impact of individual hexose transporters on glycolytic flux. Mol Microbiol 16:157–167
    [Google Scholar]
  12. Rodrigues de Sousa H., Spencer-Martins I., van Uden N. 1990; Active fructose transport in Saccharomyces sensu stricto. Taxonomic implications.. Acta Varia 5:127–134
    [Google Scholar]
  13. Sols A. 1956; Selective fermentation and phosphorylation of sugars by Sauternes yeast. biochim biophys Acta 20:62–68
    [Google Scholar]
  14. Spencer-Martins I., and van Uden N. 1985; Inactivation of active glucose transport in Candida wickerhamii is triggered by exocellular glucose. FEMS Microbiol Lett 28:277–279
    [Google Scholar]
  15. van Uden N. 1967; Transport-limited fermentation and growth of Saccharomyces cerevisiae and its competitive inhibition.. Arch Microbiol 58:155–168
    [Google Scholar]
  16. Umbreit W. W., Burris R. H., Stauffer J. F. 1964 Manometric Techniques, 4 th edn. Minneapolis: Burgess Publishing;
    [Google Scholar]
  17. Walsh M. C., Smits H. P., Schölte, M. and Van Dam K. 1994; Affinity of glucose transport in Saccharomyces cerevisiae is modulated during growth on glucose. J Bacteriol 176:953–958
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-7-1733
Loading
/content/journal/micro/10.1099/13500872-142-7-1733
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error