f Production of hybrid anthracycline antibiotics by heterologous expression of Streptomyces nogalater nogalamycin biosynthesis genes
- Authors: Kristiina Ylihonko3, Juha Hakala, Tero Kunnari, Pekka Mäntsälälä
- 3Author for correspondence: Kristiina Ylihonko. Tel: +358 21 3336856. Fax: +358 21 3336860. e-mail: firstname.lastname@example.org
- First Published Online: 01 August 1996, Microbiology 142: 1965-1972, doi: 10.1099/13500872-142-8-1965
- Subject: Genetics And Molecular Biology
- Published Online:
A cluster of anthracycline biosynthetic genes isolated from Streptomyces nogalater was expressed in Streptomyces lividans and in Streptomyces galilaeus. A 12 kb DNA fragment cloned from this cluster in pIJ486 caused the production of a novel compound when introduced into S. lividans. The compound is derived from nogalonic acid methyl ester, an early intermediate in nogalamycin biosynthesis. Complementation with the cloned 12 kb fragment of S. galilaeus mutants blocked in aclacinomycin biosynthesis caused the production of hybrid anthracyclines. Cloning of the nogalamycin gene cluster should make possible a detailed study of the biosynthesis of this interesting antibiotic, as well as the production of novel anthracyclines of potential value as cytostatic drugs.
© Society for General Microbiology 1996 | Published by the Microbiology Society
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/content/journal/micro/10.1099/13500872-142-8-1965dcterms_title,dcterms_subject-pub_serialIdent:journal/micro AND -contentType:BlogPost104
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