f Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824
- Authors: Edward M. Green, Zhuang L. Boynton, Latonia M. Harris, Frederick B. Rudolph, Eleftherios T. Papoutsakis, George N. Bennett3
- 3Author for correspondence: George N. Bennet. Tel: +1 713 527 4920. Fax: +1 713 285 5154. e-mail: firstname.lastname@example.org
- Microbiology, August 1996 142: 2079-2086, doi: 10.1099/13500872-142-8-2079
- Subject: Genetics And Molecular Biology
- Published Online:
Integrational plasmid technology has been used to disrupt metabolic pathways leading to acetate and butyrate formation in Clostridium acetobutylicum ATCC 824. Non-replicative plasmid constructs, containing either clostridial phosphotransacetylase (pta) or butyrate kinase (buk) gene fragments, were integrated into homologous regions on the chromosome. Integration was assumed to occur by a Campbell-like mechanism, inactivating either pta or buk. Inactivation of the pta gene reduced phosphotransacetylase and acetate kinase activity and significantly decreased acetate production. Inactivation of the buk gene reduced butyrate kinase activity, significantly decreased butyrate production and increased butanol production.
- Keyword(s): Clostridium acetobutylicum, acid biosynthesis, metabolic engineering, solvent biosynthesis, gene integration
© Society for General Microbiology 1996 | Published by the Microbiology Society
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