f A putative integrase gene defines the distal end of a large cluster of ToxR-regulated colonization genes in Vibrio cholerae
- Authors: Michael E. Kovach, Megan D. Shaffer, Kenneth M. Peterson2
- 2Author for correspondence: Kenneth M. Peterson. Tel: +1 318 675 5753. Fax: +1 318 675 5764. e-mail: kpeter©pop3.lsumc.edu
- Microbiology, August 1996 142: 2165-2174, doi: 10.1099/13500872-142-8-2165
- Subject: Pathogenicity And Medical Microbiology
- Published Online:
A large cluster of virulence genes encoding proteins involved in Vibrio cholerae accessory colonization factor (ACF) expression and toxin-coregulated pilus (TCP) biogenesis is flanked by sequences that resemble bacteriophage attachment (att) half-sites. Adjacent to the atfL-like site is a gene (int) that encodes a protein related to the integrase family of site-specific recombinases. The putative vibrio integrase appears to be most closely related to the Escherichia coli cryptic prophage (CP4-57) integrase protein (52% identity, 73% similarity). Genomic analysis of numerous V. cholerae strains (O1, non-O1 and O139) revealed that only vibrios capable of causing epidemic Asiatic cholera possess the TCP-ACF colonization gene cluster in association with the integrase. The fact that the integrase gene is absent in avirulent strains suggests that epidemic strains of V. cholerae obtained the TCP-ACF colonization gene cluster via horizontal transfer.
© Society for General Microbiology 1996 | Published by the Microbiology Society
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