RT Journal Article SR Electronic(1) A1 Baltazar, M. Fätima A1 Dickinson, F. Mark A1 Ratledge, ColinYR 1999 T1 Oxidation of medium-chain acyl-CoA esters by extracts of Aspergillus niger: enzymology and characterization of intermediates by HPLC JF Microbiology, VO 145 IS 1 SP 271 OP 278 DO https://doi.org/10.1099/13500872-145-1-271 PB Microbiology Society, SN 1465-2080, AB Summary: The activities of β-oxidation enzymes were measured in extracts of glucose-and triolein-grown cells of Aspergillus niger. Growth on triolein stimulated increased enzyme activity, especially for acyl-CoA dehydrogenase. No acyl-CoA oxidase activity was detected. HPLC analysis after incubation of triolein-grown cell extracts with decanoyl-CoA showed that β-oxidation was limited to one cycle. Octanoyl-CoA accumulated as the decanoyl-CoA was oxidized. β-Oxidation enzymes in isolated mitochondrial fractions were also studied. The results are discussed in the context of methyl ketone production by fungi., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-145-1-271