f Study of the glucoamylase promoter in Aspergillus niger using green fluorescent protein
- Authors: Anne L. Santerre Henriksen*, Sergine Even†, Christian Müller, Peter J. Punt, Cees A. M. J. J. van den Hondel, Jens Nielsen
- *Tel: +45 4525 2698. Fax: +45 4588 4148. e-mail: email@example.com
- Microbiology, March 1999 145: 729-734, doi: 10.1099/13500872-145-3-729
- Subject: Genomics
- Published Online:
An Aspergillus niger strain expressing a red-shifted green fluorescent protein (GFP) in the cytoplasm under the control of the glucoamylase promoter (PglaA) was characterized with respect to its physiology and morphology. Although xylose acted as a repressor carbon source during batch cultivations, PglaA-driven GFP expression by the glucoamylase promoter could be demonstrated in xylose-limited continuous cultures. In these cultivations, the xylose concentration was therefore too low to cause repression. Transient experiments initiated with a maltose pulse did not further induce red-shifted GFP production in xylose-limited continuous cultures. Maltose induction under conditions of xylose repression was microscopically observed and quantified in a flow-through chamber. Red-shifted GFP was first produced after 5 h induction. Finally the strain was characterized in glucose-limited continuous cultures, and here the area of the mycelium stained with cytoplasmic GFP increased with increasing specific growth rate, indicating that GFP can be used as a marker of cellular activity in this type of cultivation.
Present address: Department of Biotechnology, Institut National des Sciences Appliquées, F-31077 Toulouse cedex, France.
© Society for General Microbiology 1999 | Published by the Microbiology Society
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