Study of the glucoamylase promoter in Aspergillus niger using green fluorescent protein
Henriksen, Anne L. Santerre and Even, Sergine and Müller, Christian and Punt, Peter J. and van den Hondel, Cees A. M. J. J. and Nielsen, Jens,, 145, 729-734 (1999),
doi = https://doi.org/10.1099/13500872-145-3-729,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= An Aspergillus niger strain expressing a red-shifted green fluorescent protein (GFP) in the cytoplasm under the control of the glucoamylase promoter (PglaA) was characterized with respect to its physiology and morphology. Although xylose acted as a repressor carbon source during batch cultivations, PglaA-driven GFP expression by the glucoamylase promoter could be demonstrated in xylose-limited continuous cultures. In these cultivations, the xylose concentration was therefore too low to cause repression. Transient experiments initiated with a maltose pulse did not further induce red-shifted GFP production in xylose-limited continuous cultures. Maltose induction under conditions of xylose repression was microscopically observed and quantified in a flow-through chamber. Red-shifted GFP was first produced after 5 h induction. Finally the strain was characterized in glucose-limited continuous cultures, and here the area of the mycelium stained with cytoplasmic GFP increased with increasing specific growth rate, indicating that GFP can be used as a marker of cellular activity in this type of cultivation.,
language=,
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