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Volume 161, Issue 3

Genomic and phenotypic characterization of phage vB_RglS_P106B Free

Abstract

The phage P106B (vB_RglS_P106B) is a phage with a narrow spectrum of infectivity, which has been isolated from soils with a history of pea cultivation. The trapping host of P106B is an indigenous strain of (SO14B-4) isolated from soils associated with . Phenotypic characterization of the phage revealed that P106B has an approximate burst size of 21 p.f.u. per infected cell with 60 min and 100 min eclipse and latent periods, respectively. Phage P106B was unable to transduce under the conditions tested. The genome of P106B is 56 024 bp in length with a mean DNA G+C content of 47.9 %. The complete genome sequence contains 95 putative ORFs and a single tRNA gene coding for leucine with the anticodon TTA. Putative functions could only be assigned to 22 of the predicted ORFs while a significant number of ORFs (47) shared no sequence similarities to previously characterized proteins. The remaining 26 putative protein-coding genes exhibited a sequence resemblance to other hypothetical proteins. No lysogeny-related genes were found in the P106B genome.

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2015-03-01
2024-04-24
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Genomic and phenotypic characterization of Rhizobium gallicum phage vB_RglS_P106B
Microbiology 161, 611 (2015); https://doi.org/10.1099/mic.0.000022
/content/journal/micro/10.1099/mic.0.000022
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