RT Journal Article SR Electronic(1) A1 Nielsen, Signe M. A1 de Gier, Camilla A1 Dimopoulou, Chrysoula A1 Gupta, Vikas A1 Hansen, Lars H. A1 Nørskov-Lauritsen, NielsYR 2015 T1 The capsule biosynthesis locus of Haemophilus influenzae shows conspicuous similarity to the corresponding locus in Haemophilus sputorum and may have been recruited from this species by horizontal gene transfer JF Microbiology, VO 161 IS 6 SP 1182 OP 1188 DO https://doi.org/10.1099/mic.0.000081 PB Microbiology Society, SN 1465-2080, AB The newly described species Haemophilus sputorum has been cultured from the upper respiratory tract of humans and appears to have little pathogenic potential. The species encodes a capsular biosynthesis locus of approximately 12 kb composed of three distinct regions. Region I and III genes, involved in export and processing of the capsular material, show high similarity to the corresponding genes in capsulate lineages of the pathogenic species Haemophilus influenzae; indeed, standard bexA and bexB PCRs for detection of capsulated strains of H. influenzae give positive results with strains of H. sputorum. Three ORFs are present in region II of the sequenced strain of H. sputorum, of which a putative phosphotransferase showed homology with corresponding genes from H. influenzae serotype c and f. Phylogenetic analysis of housekeeping genes from 24 Pasteurellaceae species showed that H. sputorum was only distantly related to H. influenzae. In contrast to H. influenzae, the capsule locus in H. sputorum is not associated with transposases or other transposable elements. Our data suggest that the capsule locus of capsulate lineages of H. influenzae may have been recruited relatively recently from the commensal species H. sputorum by horizontal gene transfer., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000081