An iso-15 : 0 O-alkylglycerol moiety is the key structure of the E-signal in Myxococcus xanthus
Ahrendt, Tilman and Dauth, Christina and Bode, Helge B.,, 162, 138-144 (2016),
doi = https://doi.org/10.1099/mic.0.000169,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= The E-signal is one of five intercellular signals (named A- to E-signal) guiding fruiting body development in Myxococcus xanthus, and it has been shown to be a combination of the branched-chain fatty acid (FA) iso-15 : 0 and the diacylmonoalkyl ether lipid TG1. Developmental mutants HB015 (Δbkd MXAN_4265::kan) and elbD (MXAN_1528::kan) are blocked at different stages of fruiting body and spore formation as they cannot form the required iso-FA or the actual ether lipid, respectively. In order to define the structural basis of the E-signal, different mono- and triglycerides containing ether or ester bonds were synthesized and used for complementation of these mutants. Here, the monoalkylglyceride dl-1-O-(13-methyltetradecyl)glycerol exhibited comparably high levels of complementation in both mutants, restoring fruiting body and spore formation, identifying iso-15 : 0 O-alkylglycerol, part of the natural lipid TG1, as the ‘signalophore’ of E-signalling.,
language=,
type=