%0 Journal Article %A Kim, Jenny Gi Yae %A Wilson, Adam C. %T Loss of σI affects heat-shock response and virulence gene expression in Bacillus anthracis %D 2016 %J Microbiology, %V 162 %N 3 %P 564-574 %@ 1465-2080 %R https://doi.org/10.1099/mic.0.000236 %I Microbiology Society, %X The pathogenesis of Bacillus anthracis depends on several virulence factors, including the anthrax toxin. Loss of the alternative sigma factor σI results in a coordinate decrease in expression of all three toxin subunits. Our observations suggest that loss of σI alters the activity of the master virulence regulator AtxA, but atxA transcription is unaffected by loss of σI. σI-containing RNA polymerase does not appear to directly transcribe either atxA or the toxin gene pagA. As in Bacillus subtilis, loss of σI in B. anthracis results in increased sensitivity to heat shock and transcription of sigI, encoding σI, is induced by elevated temperature. Encoded immediately downstream of and part of a bicistronic message with sigI is an anti-sigma factor, RsgI, which controls σI activity. Loss of RsgI has no direct effect on virulence gene expression. sigI appears to be expressed from both the σI and σA promoters, and transcription from the σA promoter is likely more significant to virulence regulation. We propose a model in which σI can be induced in response to heat shock, whilst, independently, σI is produced under non-heat-shock, toxin-inducing conditions to indirectly regulate virulence gene expression. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000236