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Volume 162, Issue 5

The fungicidal activity of amphotericin B requires autophagy-dependent targeting to the vacuole under a nutrient-starved condition in Free

Abstract

In this study, we demonstrated that in distilled water, a nutrient-starved condition that elicits autophagy in , an array of autophagy-deficient mutants are resistant to the fungicidal effects of amphotericin B. In addition, we found that a dansyl-labelled derivative of the antibiotic colocalized with disintegrated vacuoles throughout the cytoplasm in the amphotericin B-sensitive parental strain suspended in distilled water. In contrast, the dansyl-labelled derivative was not internalized in the Δ strain, which is deficient in the formation of autophagosomes, a key early step in autophagy. However, the derivative accumulated without significant toxicity in structurally intact vacuoles in the Δ mutant, which is deficient in the degradation of autophagic bodies, the final stage in autophagy. Our data support the idea that amphotericin B can utilize autophagy-dependent trafficking into the intra-vacuolar lumen, where it interacts with the luminal leaf of the membrane to cause structurally catastrophic effects.

  • Received:
  • Accepted:
  • Published Online:
© 2016 The Authors
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2016-05-01
2024-04-19
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The fungicidal activity of amphotericin B requires autophagy-dependent targeting to the vacuole under a nutrient-starved condition in Saccharomyces cerevisiae
Microbiology 162, 848 (2016); https://doi.org/10.1099/mic.0.000269
/content/journal/micro/10.1099/mic.0.000269
/content/journal/micro/10.1099/mic.0.000269
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