RT Journal Article SR Electronic(1) A1 Leclerc, Jean-Mathieu A1 Quevillon, Eve-Lyne A1 Houde, Yoan A1 Paranjape, Kiran A1 Dozois, Charles M. A1 Daigle, FranceYR 2016 T1 Regulation and production of Tcf, a cable-like fimbriae from Salmonella enterica serovar Typhi JF Microbiology, VO 162 IS 5 SP 777 OP 788 DO https://doi.org/10.1099/mic.0.000270 PB Microbiology Society, SN 1465-2080, AB tcf (Typhi colonization factor) is one of the 12 putative chaperone/usher fimbrial clusters present in the Salmonella enterica serovar Typhi genome. We investigated the production, expression and regulation of tcf as well as its role during interaction with human cells. The tcf gene cluster was cloned and induced in Escherichia coli and S. Typhi, and the production of intertwined fibres similar to the Cbl (cable) pili of Burkholderia cepacia was observed on the bacterial surface by electron microscopy. In S. Typhi, tcf was expressed more after growth in M63 minimal medium than in standard Luria–Bertani medium. Analysis of the promoter region identified putative binding sites for the global regulators RcsB, ArgR and Fur. The expression of tcf was measured in isogenic strains lacking these global regulators. Under the conditions tested, the results showed that tcf expression was higher in the fur mutant and was regulated by iron concentration. Fur may regulate these fimbriae indirectly via the small RNAs RyhB1 and RyhB2. An isogenic mutant harbouring a deletion of the tcf cluster did not demonstrate any defect in adhesion or invasion of human epithelial cells, or in phagocytosis or survival in macrophages, when compared to the WT serovar Typhi strain. However, the tcf cluster contributed to adherence to human epithelial cells when introduced into E. coli. Thus, tcf genes encode functional fimbriae that can act as an adhesin and may contribute to colonization during typhoid fever., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000270