1887

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Volume 162, Issue 10

Novel heterologous bacterial system reveals enhanced susceptibility to DNA damage mediated by , a nearly ubiquitous and often essential gene Free

Abstract

Despite its presence in most bacteria, remains one of only 13 essential genes of unknown function in . Predictions of YqgF function often derive from sequence similarity to RuvC, the canonical Holliday junction resolvase. To clarify its role, we deleted from a bacterium where it is not essential, ADP1. Loss of impaired growth and increased the frequency of transformation and allelic replacement (TAR). When was inserted in place of its chromosomal orthologue, wild-type growth and TAR were restored. Functional similarities of in both gamma-proteobacteria were further supported by defective 16S rRNA processing by the mutant, an effect previously shown in for a temperature-sensitive allele. However, our data question the validity of deducing YqgF function strictly by comparison to RuvC. studies indicated that YqgF and RuvC can function in opposition to one another. Relative to the wild type, the Δ mutant had increased TAR frequency and increased resistance to nalidixic acid, a DNA-damaging agent. In contrast, deletion of decreased TAR frequency and lowered resistance to nalidixic acid. YqgF, but not RuvC, appears to increase bacterial susceptibility to DNA damage, including UV radiation. Nevertheless, the effects of on growth and TAR frequency were found to depend on amino acids analogous to catalytically required residues of RuvC. This new heterologous system should facilitate future investigation by exploiting the viability of mutants. In addition, bioinformatic analysis showed that a non-essential gene immediately upstream of in and () is similarly positioned in most gamma- and beta-proteobacteria. A small overlap in the coding sequences of these adjacent genes is typical. This conserved genetic arrangement raises the possibility of a functional partnership between and .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Acinetobacter , DNA damage , Holliday junction resolvase and YqgF
© 2016 The Authors
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2016-10-01
2024-04-20
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Novel heterologous bacterial system reveals enhanced susceptibility to DNA damage mediated by yqgF, a nearly ubiquitous and often essential gene
Microbiology 162, 1808 (2016); https://doi.org/10.1099/mic.0.000355
/content/journal/micro/10.1099/mic.0.000355
/content/journal/micro/10.1099/mic.0.000355
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