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Volume 162, Issue 11

Editor's Choice A feed-forward loop between SroC and MgrR small RNAs modulates the expression of and the susceptibility to polymyxin B in Typhimurium Free

Abstract

Base-pairing small RNAs (sRNAs) regulate gene expression commonly by direct interaction with cognate mRNAs. Nevertheless, recent studies have expanded this knowledge with the discovery of the RNA ‘sponges’ which are able to interact and repress the functions of classical base-pairing sRNAs. In this work, we present evidence indicating that the sponge RNA SroC from serovar Typhimurium base pairs with the MgrR sRNA, thereby antagonizing its regulatory effects on both gene expression and resistance to the antimicrobial peptide polymyxin B (PMB). By a predictive algorithm, we determined putative SroC–MgrR base-pairing regions flanking the interaction area between MgrR and its target mRNA, , encoding a LPS-modifying enzyme. With a two-plasmid system and compensatory mutations, we confirmed that SroC directly interacts and down-regulates the levels of MgrR, thus relieving the MgrR-mediated repression of mRNA. Since it was previously shown that an strain carrying an deletion is more resistant to PMB, we assessed the significance of SroC in the susceptibility of Typhimurium to PMB. Whereas the deletion increased the sensitivity to PMB, as compared to the wild-type, the resistance phenotypes between the Δ and ΔΔ strains were comparable, evidencing that mutation is epistatic to the mutation. Together, these results indicate that both SroC and MgrR sRNAs compose a coherent feed-forward loop controlling the expression and hence the LPS modification in Typhimurium.

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Keyword(s): MgrR , polymyxin B , RNA sponge , sRNAs and SroC
© 2016 The Authors
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2016-11-23
2024-04-18
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A feed-forward loop between SroC and MgrR small RNAs modulates the expression of eptB and the susceptibility to polymyxin B in Salmonella Typhimurium
Microbiology 162, 1996 (2016); https://doi.org/10.1099/mic.0.000365
/content/journal/micro/10.1099/mic.0.000365
/content/journal/micro/10.1099/mic.0.000365
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