1887

Abstract

The endonuclease tRNase Z is responsible for the 3′-end processing of tRNA precursors, which is one of the essential steps in tRNA maturation. The fission yeast contains two essential tRNase Z genes ( and ) involved in nuclear and mitochondrial tRNA 3′-end processing, respectively. Our previous studies suggest that is expressed at a very low level. Here we report characterization of the promoter. Using as a reporter, we show that the promoter contains a HomolD box and a very weak diverged TATA element. The HomolD box is usually found in the promoters of ribosomal protein genes. reporter assays suggest that the HomolD box regulates the expression of both and the ribosomal protein gene , which are arranged head-to-head on opposite strands. Overexpression of Rrn7, a candidate HomolD box-binding protein, up-regulates expression of under the control of the promoter or the promoter. Functional complementation studies suggest that the TATA-like element is essential for expression, whereas the HomolD box may play a nonessential regulatory role. We also demonstrate that a 57 nt negative regulatory element (NRE) located between the HomolD box and the TATA-like element represses the expression of under the control of the promoter. Our results suggest that the low-level expression may arise from a low level of transcription caused by lack of a strong TATA box and the NRE. Our analysis also suggests that and may be coregulated by the HomolD box.

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2017-01-01
2024-03-29
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