1887

Abstract

Diadenosine polyphosphates (ApA) are thought to act as signalling molecules regulating stress responses and biofilm formation in prokaryotes. However, ApA function in remains unknown. Here, we investigated the role of ApA in , using the wild-type and ApA hydrolase () mutant strains exposed to various stress conditions. In both wild-type and mutant cells cultured on starvation medium (CF agar), the levels of intracellular diadenosine tetraphosphate (ApA) and pentaphosphate (ApA) increased several fold during the first 16 h of development and decreased gradually thereafter. The levels of ApA and ApA in the mutant were about 5- and 11-fold higher than those in the wild-type strain at 16 h, respectively. ApA hydrolase activity of the wild-type strain increased 1.5-fold during the first 8 h of development, and it then gradually decreased. The mutant formed spores 1–2 days after the wild-type strain did, and the yield of viable spores was 5.5 % of that in the wild-type strain 5 days after inoculation onto CF agar. These results suggest the possibility that high intracellular levels of ApA and/or ApA may inhibit sporulation at the early stage of development and that the bacteria reduce intracellular ApA and ApA accumulation through ApA hydrolase activity.

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2017-01-01
2024-03-28
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